{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:41:41Z","timestamp":1760060501889,"version":"build-2065373602"},"reference-count":54,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,1,30]],"date-time":"2006-01-30T00:00:00Z","timestamp":1138579200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2007,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Diabetes mellitus is accompanied by several cardiovascular complications including atherosclerosis, cerebral ischaemia and stroke. We examined the neuroprotective effect of a 1,4\u2010dihydropyridine derivative cerebrocrast (C, a new antidiabetic agent, synthesized in the Latvian Institute of Organic Synthesis) on the level of ATP in the brain, and on changes of the EEG and ECG, as well as blood pressure parameters in anaesthetized Wistar male rats before and during 10\u2010min occlusion of both common carotid arteries. Cerebrocrast was administered i.v. at doses of 1.0 and 10\u2009\u00b5g\/kg in the v. femoralis 20\u2009min prior to ischaemia. After 10\u2010min ischaemia animals were decapitated and the brain was immediately frozen in liquid nitrogen and subsequently used for analysis of changes of ATP contention.<\/jats:p><jats:p>Cerebrocrast, administered at doses of 1.0 and 10\u2009\u00b5g\/kg 20\u2009min prior to occlusion of both common carotid arteries, completely prevented a fall in the ATP content of brain compared with the control rats. In control rats the content of ATP in brain during ischaemia decreased from 2.77\u2009\u00b1\u20090.22 (basal level) to 1.74\u2009\u00b1\u20090.20\u2009\u00b5mol\/g as a result of ischaemia. By administration of cerebrocrast 20\u2009min before occlusion of the arteries, the content of ATP in the brain remained at the level of preischaemia (1.0\u2009\u00b5g\/kg C\u2009+\u2009ischaemia 2.82\u2009\u00b1\u20090.36; 10\u2009\u00b5g\/kg C\u2009+\u2009ischaemia 2.42\u2009\u00b1\u20090.22\u2009\u00b5mol\/g). Analysis of EEG parameters both before and during 10\u2009min of occlusion showed that at a C dose of 1.0\u2009\u00b5g\/kg before occlusion produced a regular alpha rhythm during ischaemia and prevented cerebral bioelectric activity from significant changes. The depression of basal rhythm was observed at a C dose of 10\u2009\u00b5g\/kg during ischaemia in two rats out of six as well as an increase in the ECG ST segment above the isoelectric line. Blood pressure was decreased by about 10\u201320\u2009mm Hg.<\/jats:p><jats:p>We propose that pretreatment of rats with cerebrocrast at doses of 1.0 or 10\u2009\u00b5g\/kg 20\u2009min prior to ischaemia can prevent ischaemic damage of rat brain, maintain necessary energy consumption, promote ATP production in brain cells, and prevent significant changes in EEG and ECG parameters. These properties are important in diabetes mellitus and its evoked cardiovascular complications as stroke, ischaemia, etc. Copyright \u00a9 2006 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/cbf.1318","type":"journal-article","created":{"date-parts":[[2006,1,30]],"date-time":"2006-01-30T05:46:20Z","timestamp":1138599980000},"page":"203-210","source":"Crossref","is-referenced-by-count":15,"title":["Protective effect of cerebrocrast on rat brain ischaemia induced by occlusion of both common carotid arteries"],"prefix":"10.1002","volume":"25","author":[{"given":"Jan\u00efna","family":"Br\u012bede","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gun\u00e1rs","family":"Duburs","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,1,30]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-200110000-00001"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.12.6.726"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.48.2.306"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1986.97"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2004.03564.x"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410190202"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1984.tb05396.x"},{"key":"e_1_2_1_9_2","first-page":"213","article-title":"The 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