{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:49:55Z","timestamp":1760060995178,"version":"build-2065373602"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2007,5,9]],"date-time":"2007-05-09T00:00:00Z","timestamp":1178668800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2008,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The profiles of semen sialic acid and the enzyme \u03b1\u2010L\u2010fucosiadase were studied in rams undergoing chronic infection by <jats:italic>Trypanosoma congolense<\/jats:italic>. Our data showed a significant surge in the level of sialic acid with parasitaemia. The pattern followed a polynomial function we had reported for erythrocyte sialic acid in mice undergoing acute infection by <jats:italic>T. congolense<\/jats:italic>. The activity of the enzyme \u03b1\u2010fucosidase decreased progressively with approximately 60% decrease at the end of the 14 weeks of infection. Representative semen samples from the control and infected rams were subjected to kinetic characterization. While the uninfected semen sample showed two active pH peaks at 4.5\u20135.5 and at 6.8\u20137.2, respectively, there was an apparent shift to only a single pH optimum at 4.5\u20135.5 for the pathological semen. The fucosidases from both sources were optimally active at 35\u00b0C albeit with contrasting activation energies (<jats:italic>E<\/jats:italic><jats:sub>a<\/jats:sub>) with values 20.58 and 35\u2009kJ\/mol for the control and infected semen, respectively. Kinetic studies using methylumbelliferyl\u2010\u03b2\u2010fucoside (4MU\u2010Fuc) as substrate gave <jats:italic>K<\/jats:italic><jats:sub>M<\/jats:sub> and <jats:italic>V<\/jats:italic><jats:sub>max<\/jats:sub> values of 3.25\u2009\u00b5M and 14.6\u2009\u00b5mol.\u2009min<jats:sup>\u22121<\/jats:sup>\u2009mg<jats:sup>\u22121<\/jats:sup>, respectively for the control semen. The values for the infected semen were 18.25\u2009\u00b5M and 10.5\u2009\u00b5mol.\u2009min<jats:sup>\u22121<\/jats:sup>\u2009mg<jats:sup>\u22121<\/jats:sup>, respectively. The significance of these results is discussed as they relate to loss in reproductive capacity in trypanosomoses. Copyright \u00a9 2007 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/cbf.1415","type":"journal-article","created":{"date-parts":[[2007,5,9]],"date-time":"2007-05-09T06:22:00Z","timestamp":1178691720000},"page":"154-161","source":"Crossref","is-referenced-by-count":0,"title":["Semen sialic acid surge and modulation of \u03b1\u2010<scp>L<\/scp>\u2010fucosidase activity: possible link to loss in reproductive capacity during trypanosomiasis"],"prefix":"10.1002","volume":"26","author":[{"given":"Okubanjo O.","family":"Oluyinka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inuwa H.","family":"Mairo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph A.","family":"Ajanusi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ogwu","family":"David","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor","family":"Sekoni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew J.","family":"Nok","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,5,9]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvg093"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1016\/S0034-5288(18)32398-1","article-title":"Delay in resolution of trypanosome induced genital lesions in male rabbit infected with Trypanosoma brucei and treated with diminazene aceturate","volume":"32","author":"Ikede BO","year":"1982","journal-title":"Res Vet Sci"},{"key":"e_1_2_1_4_2","first-page":"168","article-title":"The pathogenesis of experimentally induced Trypanosoma brucei infection in the dog","volume":"102","author":"Morrison WI","year":"1981","journal-title":"Am J Pathol"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4320(86)90111-9"},{"key":"e_1_2_1_6_2","first-page":"355","article-title":"Production performance of an imported herd of Friesian cattle subject to trypanosome challenge in the humid lowlands tropics of Nigeria","volume":"4","author":"Breinholt KA","year":"1983","journal-title":"East Afric Agric J"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02239926"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(94)90373-Q"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9975(75)90082-1"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(92)90098-T"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(90)90112-O"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9975(89)90002-9"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.6576"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.2108\/zsj.19.43"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(86)90333-5"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/1098-2795(200010)57:2<194::AID-MRD11>3.0.CO;2-0"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2318(08)60109-2"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(85)90112-4"},{"key":"e_1_2_1_19_2","first-page":"161","article-title":"Influence of wet fixation, staining techniques and storage time on bulls sperm morphology","volume":"33","author":"Sekoni VO","year":"1981","journal-title":"Nord Vet Med"},{"key":"e_1_2_1_20_2","unstructured":"ChemineauP CagnieY.1991;Training Manual on Artificial Insemination in Sheep and Goats.FAO Animal Production and Health Paper:83."},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0965-1748(02)00031-0"},{"key":"e_1_2_1_22_2","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1042\/bj0810384","article-title":"Methods for the quantitative estimation of N\u2010acetyyl neuraminic acids and their application to hydrolysates of sialomucoids","volume":"81","author":"Aminof D","year":"1961","journal-title":"Biochem J"},{"key":"e_1_2_1_23_2","first-page":"105","article-title":"Structure and function of mammalian \u03b1\u2010L\u2010fucosidases","volume":"4","author":"Alhadeff JA","year":"1998","journal-title":"Trends Comp Biochem Physiol"},{"key":"e_1_2_1_24_2","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/S0021-9258(19)45753-0","article-title":"Purification and properties of an \u03b1\u2010L\u2010fucosidase from rat epididymis","volume":"247","author":"Carlsen RB","year":"1972","journal-title":"J. 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