{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T14:24:41Z","timestamp":1789741481145,"version":"4.0.1"},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2010,7,13]],"date-time":"2010-07-13T00:00:00Z","timestamp":1278979200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2010,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An 11\u03b2\u2010hydroxysteroid dehydrogenase type 1 (11\u03b2\u2010HSD1) produces glucocorticoid (GC) from 11\u2010keto metabolite, and its modulation has been suggested as a novel approach to treat metabolic diseases. In contrast, type 2 isozyme 11\u03b2\u2010HSD2 is involved in the inactivation of glucocorticoids (GCs), protecting the non\u2010selective mineralocorticoid receptor (MR) from GCs in kidney. Therefore, when 11\u03b2\u2010HSD1 inhibitors are pursued to treat the metabolic syndrome, preferential selectivity of inhibitors for type 1 over type 2 isozyme is rather important than inhibitory potency. Primarily, to search for cell lines with 11\u03b2\u2010HSD2 activity, we investigated the expression profiles of enzymes or receptors relevant to GC metabolism in breast, colon, and bone\u2010derived cell lines. We demonstrated that MCF\u20107 cells had high expression for 11\u03b2\u2010HSD2, but not for 11\u03b2\u2010HSD1 with its cognate receptor. Next, for the determination of enzyme activity indirectly, we adopted homogeneous time resolved fluorescence (HTRF) cortisol assay. Obviously, the feasibility of HTRF to cellular 11\u03b2\u2010HSD2 was corroborated by constructing inhibitory response to an 11b\u2010HSD2 inhibitor glycyrrhetinic acid (GA). Taken together, MCF\u20107 that overexpresses type 2 but not type 1 enzyme is chosen for cellular 11\u03b2\u2010HSD2 assay, and our results show that a nonradioactive HTRF assay is applicable for type 2 as well as type 1 isozyme. Copyright \u00a9 2010 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/cbf.1674","type":"journal-article","created":{"date-parts":[[2010,7,13]],"date-time":"2010-07-13T04:43:36Z","timestamp":1278996216000},"page":"440-447","source":"Crossref","is-referenced-by-count":3,"title":["Selection and optimization of MCF\u20107 cell line for screening selective inhibitors of 11\u03b2\u2010hydroxysteroid dehydrogenase 2"],"prefix":"10.1002","volume":"28","author":[{"given":"Chi Hyun","family":"Kim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young Sik","family":"Cho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,7,13]]},"reference":[{"key":"e_1_2_7_2_2","first-page":"141","article-title":"Glucocorticoids and 11beta\u2010hydroxysteroid dehydrogenase type 1 in obesity and the metabolic syndrome","volume":"32","author":"Stimson RH","year":"2007","journal-title":"Minerva Endocrinol"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.icb.7100034"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1367.012"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2005.10.001"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/stke.3042005pe48"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2002-020048"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2009.05.006"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2001.16.6.1037"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.83.4.1325"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1610455"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e31815f2362"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-007-9504-9"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1530\/eje.1.02121"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(97)00299-X"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-7-39"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1530\/eje.1.01954"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(98)00256-1"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3131007"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2009.04.038"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12033-008-9050-y"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2003.11.012"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2000.00976.x"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2265.1997.1230939.x"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.105.539122"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1677\/joe.1.06019"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.86.6.2728"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2004-825724"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.30.403"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2009.02.010"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.2005-1626"}],"container-title":["Cell Biochemistry and Function"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcbf.1674","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbf.1674","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:17:39Z","timestamp":1760055459000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/cbf.1674"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,7,13]]},"references-count":30,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2010,8]]}},"alternative-id":["10.1002\/cbf.1674"],"URL":"https:\/\/doi.org\/10.1002\/cbf.1674","archive":["Portico"],"relation":{},"ISSN":["0263-6484","1099-0844"],"issn-type":[{"value":"0263-6484","type":"print"},{"value":"1099-0844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,7,13]]}}}