{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T11:05:54Z","timestamp":1761217554013,"version":"build-2065373602"},"reference-count":19,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,5,16]],"date-time":"2012-05-16T00:00:00Z","timestamp":1337126400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:p>Bone marrow\u2010derived mesenchymal stem cells (BM\u2010MSCs ) transplantation has been reported to be a promising therapy for myocardial infarction (MI). However, low survival rate of BM\u2010MSCs in infarcted heart is one of the major limitations for the perspective clinical application. In this study, we aimed to investigate the effect of hepatocyte growth factor (HGF) on left ventricular function improvement of HGF gene\u2010modified BM\u2010MSCs (HGF\u2010MSCs) after its delivery into the infarcted rat hearts. BM\u2010MSCs were isolated with fibroblast\u2010like morphology and expressed CD44+CD29+CD90+\/CD34\u2010CD45\u2010CD31\u2010CD11a. After 5\u2010azacytidine induction <jats:italic>in vitro<\/jats:italic>, 20%\u201330% of the cells were positively stained for desmin, cardiac\u2010specific cardiac troponin I and connexin\u201043. Histological staining revealed that 2\u2009weeks after MI is an optimal time point with decreased neutrophil infiltration and increased vascular number. Minimal infarct size and best haemodynamic analysis were also observed after cell injection at 2\u2009weeks compared with that of 1\u2009h, 1\u2009week or 4\u2009weeks. Echocardiogram confirmed that transplantation with HGF\u2010MSCs significantly improved left ventricular function compared with other groups in rat MI models. MSCs and HGF\u2010MSCslabelled with DAPI were detected 4\u2009weeks after MI in the infarcted area. Decreased infarcted scar area and increased angiogenesis formation could be found in HGF\u2010MSCs group than in other groups as demonstrated by hematoxylin and eosin (H&amp;E) staining and factor VIII staining. These results indicate that HGF\u2010MSCs transplantation could enhance the contractile function and attenuate left ventricular remodelling efficiently in rats with MI. Copyright \u00a9 2012 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/cbf.2836","type":"journal-article","created":{"date-parts":[[2012,5,17]],"date-time":"2012-05-17T10:10:55Z","timestamp":1337249455000},"page":"574-581","source":"Crossref","is-referenced-by-count":19,"title":["Effects of hepatocyte growth factor overexpressed bone marrow\u2010derived mesenchymal stem cells on prevention from left ventricular remodelling and functional improvement in infarcted rat hearts"],"prefix":"10.1002","volume":"30","author":[{"given":"Shenxu","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Cardiology, Xijing Hospital Fourth Military Medical University  Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xing","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Xijing Hospital Fourth Military Medical University  Xi'an 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