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The study was based on definitive histological assessment of liver damage caused by CCl<jats:sub><jats:italic>4<\/jats:italic><\/jats:sub> in the presence and absence of PM: PM (78 \u03bcmol kg<jats:sup>\u2212<jats:italic>1<\/jats:italic><\/jats:sup>, i.p.) protected against CCl<jats:sub><jats:italic>4<\/jats:italic><\/jats:sub>\u2010induced hepatic necrosis 24 h after a low dose of CCl<jats:sub><jats:italic>4<\/jats:italic><\/jats:sub> (1.3 mmol kg<jats:sup>\u2212<jats:italic>1<\/jats:italic><\/jats:sup>) but not against a higher dose (13.0 mmol kg<jats:sup>\u2212<jats:italic>1<\/jats:italic><\/jats:sup>). The large increases in plasma activities of GOT, GPT and LDH produced by dosing with CCl<jats:sub><jats:italic>4<\/jats:italic><\/jats:sub> were partially inhibited by the administration of PM. PM and CCl<jats:sub><jats:italic>4<\/jats:italic><\/jats:sub> caused a synergistic and long\u2010lasting decrease in body temperature (2\u20133\u00b0C for 8\u201310 h). Modifying the toxicity with PM, together with a low dose of CCl<jats:sub><jats:italic>4<\/jats:italic><\/jats:sub>, helped to minimize secondary effects of CCl<jats:sub><jats:italic>4<\/jats:italic><\/jats:sub>, to clarify the sequence of toxic events, and to assess the sensitivity of some standard tests of hepatotoxicity. Simultaneous measurement of over 20 commonly used biochemical screening tests in individual animals 3 or 6 h after treatment permitted direct correlation of a wide variety of concentrations, activities and effects. For example, liver CHCl<jats:sub><jats:italic>3<\/jats:italic><\/jats:sub> concentrations (as a measure of CCl<jats:sub><jats:italic>4<\/jats:italic><\/jats:sub> metabolism) correlate strongly with increases in diene conjugation of microsomal lipids (as a measure of CCl<jats:sub><jats:italic>4<\/jats:italic><\/jats:sub>\u2010induced lipid peroxidation); malonaldehyde production appears to be less sensitive as a measure of lipid peroxidation in vivo than diene conjugation. The changes induced in each parameter and the correlations between them are discussed with reference to the overall nature of the hepatotoxic reaction and its modification by PM.<\/jats:p>","DOI":"10.1002\/cbf.290010110","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T10:04:23Z","timestamp":1117274663000},"page":"55-63","source":"Crossref","is-referenced-by-count":9,"title":["Correlations between common tests for assessment of liver damage: Indices of the hepatoprotective activity of promethazine in carbon tetrachloride hepatotoxicity"],"prefix":"10.1002","volume":"1","author":[{"given":"Colin J.","family":"Reddrop","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin H.","family":"Cheeseman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. 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