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To determine if this was due to a decreased amount of ATP translocase in the mitochondrial membranes, or a structural modification of this protein, an electrophoretic study was undertaken. Total proteins and purified translocase preparations from (NP) and (HP) mitochondria were analysed by SDS gel electrophoresis. In the two type of mitochondria no significant differences were observed either in the amount of ATP translocase or in the molecular weight. Also, neither nonequilibrium pH gradient gel electrophoresis nor the analysis of peptides produced by limited proteolysis revealed any structural difference between the two types of protein. On the basis of these results, the depressed translocase activity observed in (HP) mitochondria cannot be explained by a reduced amount of the nucleotide translocase, nor a structural alteration of this protein. Possible inhibition of (HP) translocase activity by Ca<jats:sup>2+<\/jats:sup> accumulation or by other mechanisms is discussed.<\/jats:p>","DOI":"10.1002\/cbf.290090307","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T10:45:28Z","timestamp":1117277128000},"page":"193-199","source":"Crossref","is-referenced-by-count":1,"title":["Mitochondrial nucleotide translocase from skeletal muscle of halothane sensitive pigs: An electrophoretic study"],"prefix":"10.1002","volume":"9","author":[{"given":"S.","family":"Ayoub","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Berson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Rock","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Younes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,17]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_1_2_2","DOI":"10.1007\/978-1-4613-2079-1_14"},{"doi-asserted-by":"publisher","key":"e_1_2_1_3_2","DOI":"10.1038\/225653a0"},{"doi-asserted-by":"publisher","key":"e_1_2_1_4_2","DOI":"10.1007\/BF03004695"},{"key":"e_1_2_1_5_2","first-page":"173","volume-title":"Recent Advances in Analgesia","author":"Ellis F. 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