{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:04:44Z","timestamp":1760141084251,"version":"build-2065373602"},"reference-count":48,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,2,17]],"date-time":"2004-02-17T00:00:00Z","timestamp":1076976000000},"content-version":"vor","delay-in-days":3821,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[1993,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The biosynthesis of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) in monocyte\u2010like leukemia U937 cells was monitored by adding [<jats:sup>3<\/jats:sup>H]choline, [<jats:sup>14<\/jats:sup>C]ethanolamine or [<jats:sup>14<\/jats:sup>C]glycerol to the culture media; incorporation into phospholipid (PL) increased with time. The effect of unsaturated fatty acids (UFA) on PC and PE synthesis was investigated by pretreating U937 cells for 72h with 10 \u03bc<jats:sc>M<\/jats:sc> 18:1 (<jats:italic>n<\/jats:italic> \u20139), 18:2 (<jats:italic>n<\/jats:italic> \u20136), 18:3 (<jats:italic>n<\/jats:italic> \u20133), 20:4 (<jats:italic>n<\/jats:italic> \u20136) and 20:5 (<jats:italic>n<\/jats:italic> \u20133). The UFA caused no alteration in cell growth, as evidenced by light microscopy and the incorporation of [<jats:sup>3<\/jats:sup>H]thymidine and [<jats:sup>3<\/jats:sup>H]leucine. Total cellular uptake of radioactive precursors remained unaffected by all the treatments. Pretreatment with 20:5 resulted in approximately 25 per cent reduction in the incorporation of [<jats:sup>3<\/jats:sup>H]choline into PL, while no significant effect was detected with the other UFAs. 18:3, 20:4 and 20:5 depressed the incorporation of [<jats:sup>14<\/jats:sup>C]ethanolamine into PL by 34 per cent, 28 per cent and 49 per cent respectively. However, there was no redistribution of label with any of the treatments. 18:3, 20:4 and 20:5 also antagonized the stimulatory effect of endotoxin (LPS) on PC and PE synthesis. In addition, the incorporation from [<jats:sup>14<\/jats:sup>C]glycerol into PC and PE was reduced by 18:3, 20:4 and 20:5.<\/jats:p><jats:p>Although the PL composition of the cells remained essentially unaffected, our study shows that chronic treatment of U937 cells with <jats:italic>n<\/jats:italic> \u20133 PUFA (20:5) depressed PC and PE synthesis, and 18:3 and 20:4 also caused inhibition of PE synthesis.<\/jats:p>","DOI":"10.1002\/cbf.290110308","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T10:53:15Z","timestamp":1117277595000},"page":"201-209","source":"Crossref","is-referenced-by-count":5,"title":["Differential effects of unsaturated fatty acids on phospholipid synthesis in human leukemia monocytic U937 cells"],"prefix":"10.1002","volume":"11","author":[{"given":"Arthur J.","family":"Chu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camtu T.","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Moore","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)34276-0"},{"key":"e_1_2_1_3_2","first-page":"1","volume-title":"Advances in Membrane Fluidity","author":"Curtain C. 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