{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T10:29:08Z","timestamp":1761647348571,"version":"build-2065373602"},"reference-count":17,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,2,17]],"date-time":"2004-02-17T00:00:00Z","timestamp":1076976000000},"content-version":"vor","delay-in-days":3730,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[1993,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We studied the effect of PTH (10\u2013100 nM) on transductive mechanisms (adenylate cyclase activity, Ca<jats:sup>2+<\/jats:sup> metabolism, IP<jats:sub>3<\/jats:sub> levels) in cell cultures derived from normal and otosclerotic human bone fragments. The cultured cells were osteoblast\u2010like but with calcitonin\u2010receptors still present and with PTH receptors coupled with the adenylate cyclase system.<\/jats:p><jats:p>The results showed that PTH activated adenylate cyclase and increased the intracellular Ca<jats:sup>2+<\/jats:sup> levels with qualitative and quantitative differences between the two cellular populations. In particular, otosclerotic cells responded less to hormone stimulation, which is in accord with the current hypothesis of a desensitization of the receptor\/enzyme complex associated with the pathological status.<\/jats:p>","DOI":"10.1002\/cbf.290110406","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T10:55:41Z","timestamp":1117277741000},"page":"257-261","source":"Crossref","is-referenced-by-count":9,"title":["PTH induces modification of transductive events in otosclerotic bone cell cultures"],"prefix":"10.1002","volume":"11","author":[{"given":"G.","family":"Fan\u00f3","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Venti\u2010Donti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Belia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Paludetti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Antonica","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Donti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Maurizi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.290030304"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650010202"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-128-2-1021"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711450403"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1987.252.1.E44"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0196-0709(88)80010-3"},{"key":"e_1_2_1_8_2","unstructured":"Bodo M. Venti G. Pezzetti F. Ardisia G. Antonica A. Corinci F.andBecchetti E.(1992). Interleukin 1\u2010alfa: regulation of cellular proliferation and biosynthetic activities in cultured osteoblast\u2010like cells.Cell. Mol. Biol. in press."},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1177\/000348948609500316"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0303-7207(89)90120-2"},{"key":"e_1_2_1_11_2","first-page":"35","article-title":"Adenylate cyclase assay","volume":"10","author":"Salomon Y.","year":"1979","journal-title":"Adv. Cyc. Nuc. Res."},{"key":"e_1_2_1_12_2","first-page":"657","article-title":"Fc receptor\u2010mediated phagocitosis occurs in macrophages at exceedingly low cytosolic Ca2+ levels","volume":"106","author":"Di Virgilio F.","year":"1988","journal-title":"J. Biol. 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J.","year":"1989"},{"key":"e_1_2_1_16_2","first-page":"3","volume-title":"Physiology and Pharmacology of Transmembrane Signalling","author":"Ui M.","year":"1989"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2536957"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2740015"}],"container-title":["Cell Biochemistry and Function"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcbf.290110406","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbf.290110406","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T23:46:28Z","timestamp":1760053588000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/cbf.290110406"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,12]]},"references-count":17,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1993,12]]}},"alternative-id":["10.1002\/cbf.290110406"],"URL":"https:\/\/doi.org\/10.1002\/cbf.290110406","archive":["Portico"],"relation":{},"ISSN":["0263-6484","1099-0844"],"issn-type":[{"type":"print","value":"0263-6484"},{"type":"electronic","value":"1099-0844"}],"subject":[],"published":{"date-parts":[[1993,12]]}}}