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Lipid phosphorylation <jats:italic>in vitro<\/jats:italic> showed the presence of diacylglycerol (DAG)\u2010, phosphatidylinositol (PI)\u2010 and phosphatidylinositol\u20104\u2010phosphate (PIP)\u2010kinase activity, with differences between total tissue homogenates and isolated nuclei, relevant to the treatment <jats:italic>in vivo<\/jats:italic>. Administration of hydrocortisone (HC) produced a marked decrease in the phosphorylated nuclear products without influencing the homogenate kinase activity. Under conditions which were optimal for the kinase activities, nuclear PIP\u2010kinase was strongly increased in presence of a high blood level of HC whereas PI\u2010kinase activity was reduced. From these observations it appears that the observed differences were due to specific modulation of kinase activities rather than to changes in the availability of substrates.<\/jats:p><jats:p>The phosphoinositide\u2010specific phospholipase C (PLC) activity was also investigated. In the presence of a high HC blood level, the phosphodiesteratic cleavage of PIP strongly increased, while that of phosphatidylinositol bisphosphate (PIP<jats:sub>2<\/jats:sub>) was similar in normal and hypercortisolemic conditions. Nuclear phosphoinositide hydrolysis was affected by PLC, \u03b2 and \u03b3 isoforms, which were equally represented in all the conditions investigated, indicating that the observed changes of activity were due to a modulation rather than to a change in the amount of enzyme.<\/jats:p><jats:p>These results suggest that inositol lipid metabolism plays a role in the nuclear modifications accompanying steroid hormone induction of transcriptional activity.<\/jats:p>","DOI":"10.1002\/cbf.290120308","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T11:01:24Z","timestamp":1117278084000},"page":"201-207","source":"Crossref","is-referenced-by-count":5,"title":["Inositol lipid phosphorylation and breakdown in rat liver nuclei is affected by hydrocortisone blood levels"],"prefix":"10.1002","volume":"12","author":[{"given":"Patrizia","family":"Santi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Marmiroli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Falcieri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Bertagnolo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Capitani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,17]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"463","article-title":"Role of specific chromosomal proteins and DNA sequences in the nuclear binding sites for steroid receptors","volume":"39","author":"Spelsberg T. 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