{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T07:10:12Z","timestamp":1772003412211,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,2,17]],"date-time":"2004-02-17T00:00:00Z","timestamp":1076976000000},"content-version":"vor","delay-in-days":3091,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[1995,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The action of cyanide (500 \u03bc<jats:sc>M<\/jats:sc>), 2,4\u2010dinitrophenol (50 \u03bc<jats:sc>M<\/jats:sc>) and atractyloside (100 \u03bc<jats:sc>M<\/jats:sc>) on glycogen catabolism and oxygen uptake was investigated in the bivascularly perfused liver of fed rats. Cyanide, 2,4\u2010dinitrophenol and atractyloside were infused at identical rates into the hepatic artery in either the anterograde or retrograde perfusion. The accessible aqueous cell spaces were determined by means of the multiple\u2010indicator dilution technique. Glucose release, oxygen uptake and glycolysis were measured as metabolic parameters.<\/jats:p><jats:p>Oxygen uptake changes per unit cell space caused by atractyloside (inhibition) and 2,4\u2010dinitrophenol (stimulation) were equal in the retrograde perfusion (periportal cells) and the anterograde perfusion (space enriched in perivenous cells); the decreases caused by cyanide were higher in the retrograde perfusion. Glucose release from periportal cells was not increased upon inhibition of oxidative phosphorylation, a phenomenon which was independent of the mechanism of action of the inhibitor. There were nearly identical changes in glycolysis in the periportal and perivenous cells.<\/jats:p><jats:p>It was concluded that: (1) oxygen concentration in the perfused rat liver, if maintained above 100 \u03bc<jats:sc>M<\/jats:sc>, had little influence on the zonation of the respiratory activity; (2) in spite of the lower activities of the key enzymes of glycolysis in the periportal hepatocytes, as assayed under standard conditions, these cells were as effective as the perivenous ones in generating ATP in the cytosol when oxidative phosphorylation was impaired; (3) the key enzymes of glycogenolysis and glycolysis in periportal and perivenous cells responded differently to changes in the energy charge.<\/jats:p>","DOI":"10.1002\/cbf.290130311","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T11:02:21Z","timestamp":1117278141000},"page":"201-209","source":"Crossref","is-referenced-by-count":13,"title":["Bivascular liver perfusion in the anterograde and retrograde modes: Zonation of the response to inhibitors of oxidative phosphorylation"],"prefix":"10.1002","volume":"13","author":[{"given":"Jorgete","family":"Constantin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emy","family":"Ishii\u2010Iwamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fumie","family":"Suzuki\u2010Kemmelmeier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nair S.","family":"Yamamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adelar","family":"Bracht","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1016\/S0022-3565(25)13420-4","article-title":"Combined hepatic arterial\u2013portal venous and hepatic arterial\u2013hepatic venous perfusion to probe the abundance of drug metabolizing activities: perihepatic venous O\u2010deethylation activity for phenacetin and periportal sulfation activity for acetaminophen in the once\u2010through rat liver preparation","volume":"247","author":"Pang K. 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