{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:47:58Z","timestamp":1760057278200,"version":"build-2065373602"},"reference-count":21,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,6,2]],"date-time":"2014-06-02T00:00:00Z","timestamp":1401667200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:p>The present study deals with the possible effects of selected environmental agents upon the uptake and metabolism of <jats:sc>d<\/jats:sc>\u2010glucose in isolated acinar and ductal cells from the rat submandibular salivary gland. In acinar cells, the uptake of <jats:sc>d<\/jats:sc>\u2010[U\u2010<jats:sup>14<\/jats:sup>C]glucose and its non\u2010metabolised analogue 3\u2010<jats:italic>O<\/jats:italic>\u2010[<jats:sup>14<\/jats:sup>C\u2010methyl]\u2010<jats:sc>d<\/jats:sc>\u2010glucose was not affected significantly by phloridzin (0.1\u2009mM) or substitution of extracellular NaCl (115\u2009mM) by an equimolar amount of CsCl, whilst cytochalasin B (20\u2009\u03bcM) decreased significantly such an uptake. In ductal cells, both phloridzin and cytochalasin B decreased the uptake of <jats:sc>d<\/jats:sc>\u2010glucose and 3\u2010<jats:italic>O<\/jats:italic>\u2010methyl\u2010<jats:sc>d<\/jats:sc>\u2010glucose. Although the intracellular space was comparable in acinar and ductal cells, the catabolism of <jats:sc>d<\/jats:sc>\u2010glucose (2.8 or 8.3\u2009mM) was two to four times higher in ductal cells than in acinar cells. Phloridzin (0.1\u2009mM), ouabain (1.0\u2009mM) and cytochalasin B (20\u2009\u03bcM) all impaired <jats:sc>d<\/jats:sc>\u2010glucose catabolism in ductal cells. Such was also the case in ductal cells incubated in the absence of extracellular Ca<jats:sup>2+<\/jats:sup> or in media in which NaCl was substituted by CsCl. It is proposed that the ductal cells in the rat submandibular gland are equipped with several systems mediating the insulin\u2010sensitive, cytochalasin B\u2010sensitive and phloridzin\u2010sensitive transport of <jats:sc>d<\/jats:sc>\u2010glucose across the plasma membrane. 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