{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T03:47:35Z","timestamp":1783828055532,"version":"3.55.0"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,12,16]],"date-time":"2014-12-16T00:00:00Z","timestamp":1418688000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"a grant from the Korea Healthcare Technology R&D Project, Ministry for Health, Welfare & Family Affairs, Republic of Korea","award":["A120203"],"award-info":[{"award-number":["A120203"]}]},{"name":"by a grant from the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology","award":["NRF-2010-0010431"],"award-info":[{"award-number":["NRF-2010-0010431"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:p>Engraft cells are often exposed to oxidative stress and inflammation; therefore, any factor that can provide the stem cells resistance to these stresses may yield better efficacy in stem cell therapy. Studies indicate that histone deacetylase (HDACs) inhibitors alleviate damage induced by oxidative stress. In this study, we investigated whether regulation of reactive oxygen species (ROS) occurs through the HDAC inhibitor trichostatin A (TSA) in human bone marrow\u2010mesenchymal stem cells (hBM\u2010MSCs). Intracellular ROS levels increased following exposure to hydrogen peroxide (H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>), and were suppressed by TSA treatment. Levels of the antioxidant enzyme superoxide dismutase 2 (SOD2) increased following treatment with 200\u2009nM TSA and to a lesser level at 1\u20135\u2009\u03bcM TSA. Cell protective effects against oxidative stress were significantly increased in TSA\u2010MSCs after treatment with low doses of TSA (50\u2013500\u2009nM) and decreased with high doses of TSA (5\u201310\u2009\u03bcM). Consistent results were obtained with immunoblot analysis for caspase3. Investigation of Forkhead box O1 (FOXO1), superoxide dismutase 2 (SOD2), and p53 levels to determine intracellular signaling by TSA in oxidative stress\u2010induced MSCs demonstrated that expression of phosphorylated\u2010FOXO1 and phosphorylated\u2010SOD2 decreased in H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010treated MSCs while levels of p53 increased. These effects were reversed by the treatment of 200\u2009nM TSA. These results suggest that the main function of ROS modulation by TSA is activated through SOD2 and FOXO1. Thus, optimal treatment with TSA may protect hBM\u2010MSCs against oxidative stress. Copyright \u00a9 2014 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/cbf.3084","type":"journal-article","created":{"date-parts":[[2014,12,16]],"date-time":"2014-12-16T07:25:50Z","timestamp":1418714750000},"page":"37-43","source":"Crossref","is-referenced-by-count":16,"title":["Trichostatin a modulates intracellular reactive oxygen species through SOD2 and FOXO1 in human bone marrow\u2010mesenchymal stem cells"],"prefix":"10.1002","volume":"33","author":[{"given":"Sin\u2010Gu","family":"Jeong","sequence":"first","affiliation":[{"name":"Department of Biology College of Natural Science, Chosun University  Gwangju Korea"},{"name":"Department of Life Science, BK21\u2010Plus Research Team for Bioactive Control Technology Chosun University  Gwangju Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Goang\u2010Won","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Biology College of Natural Science, Chosun University  Gwangju Korea"},{"name":"Department of Life Science, BK21\u2010Plus Research Team for Bioactive Control Technology Chosun University  Gwangju Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,12,16]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.1100090504"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2013.08.059"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0001272"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2009.0453"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI20761"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05862"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000124476.32871.E3"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000039526.42991.93"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000231289.63468.08"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0052700"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.302"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2007.9.1"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/608478"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2005.07.006"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.001222"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3109\/01480545.2014.951762"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2133"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1779"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-5949.2010.00203.x"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/emmm.201201923"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2007.11.031"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198003203021204"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn1430"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3223(00)01020-9"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3223(00)00950-1"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.2174\/0929867043365099"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2011.06.003"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuro.2013.03.007"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0050235"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.3727\/096368910X507204"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1112125"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2012.01.001"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2011.01492.x"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0018087"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2012.1705"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2007.01.033"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0001670"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.13.11.1385"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2008.01.011"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2008.10.011"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1002178107"}],"container-title":["Cell Biochemistry and Function"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcbf.3084","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbf.3084","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T19:44:34Z","timestamp":1760039074000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/cbf.3084"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,12,16]]},"references-count":41,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,1]]}},"alternative-id":["10.1002\/cbf.3084"],"URL":"https:\/\/doi.org\/10.1002\/cbf.3084","archive":["Portico"],"relation":{},"ISSN":["0263-6484","1099-0844"],"issn-type":[{"value":"0263-6484","type":"print"},{"value":"1099-0844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,12,16]]}}}