{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T04:02:42Z","timestamp":1780372962880,"version":"3.54.1"},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,2,6]],"date-time":"2015-02-06T00:00:00Z","timestamp":1423180800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:p>Adenylate kinase plays an important role in cellular energy homeostasis by catalysing the interconversion of adenine nucleotides. The goal of present study was to evaluate the contribution of the adenylate kinase reaction to oxidative ATP synthesis by direct measurements of ATP using <jats:sup>31<\/jats:sup>P NMR spectroscopy. Results show that AMP can stimulate ATP synthesis in the presence or absence of ADP. In particular, addition of 1\u2009mM AMP to the 0.6\u2009mM ADP superfusion system of isolated superfused mitochondria (contained and maintained in agarose beads) led to a 25% increase in ATP synthesis as measured by the increase in <jats:italic>\u03b2<\/jats:italic>ATP signal. More importantly, we show that AMP can support ATP synthesis in the absence of ADP, demonstrated as follows. Superfusion of mitochondria without ADP led to the disappearance of ATP <jats:italic>\u03b3<\/jats:italic>, <jats:italic>\u03b1<\/jats:italic> and <jats:italic>\u03b2<\/jats:italic> signals and the increase of P<jats:sub>i<\/jats:sub>. Addition of AMP to the medium restored the production of ATP, as demonstrated by the reappearance of <jats:italic>\u03b3<\/jats:italic>, <jats:italic>\u03b1<\/jats:italic> and <jats:italic>\u03b2<\/jats:italic> ATP signals, in conjunction with a decrease in P<jats:sub>i<\/jats:sub>, which is being used for ATP synthesis. Polarographic studies showed Mg<jats:sup>2+<\/jats:sup> dependence of this process, confirming the specificity of the adenylate kinase reaction. Furthermore, data obtained from this study demonstrate, for the first time, that different aspects of the adenylate kinase reaction can be evaluated with <jats:sup>31<\/jats:sup>P NMR spectroscopy. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p><jats:p>SIGNIFICANCE OF RESEARCH PARAGRAPH<\/jats:p><jats:p>The data generated in the present study indicate that <jats:sup>31<\/jats:sup>P NMR spectroscopy can effectively be used to study the adenylate kinase reaction under a variety of conditions. This is important because understanding of adenylate kinase function and\/or malfunction is essential to understanding its role in health and disease. The data obtained with <jats:sup>31<\/jats:sup>P NMR were confirmed by polarographic studies, which further strengthens the robustness of the NMR findings. In summary, <jats:sup>31<\/jats:sup>P NMR spectroscopy provides a sensitive tool to study adenylate kinase activity in different physiological and pathophysiological conditions, including but not exclusive of, cancer, ischemic injury, hemolytic anemia and neurological problems such as sensorineural deafness.<\/jats:p>","DOI":"10.1002\/cbf.3089","type":"journal-article","created":{"date-parts":[[2015,2,6]],"date-time":"2015-02-06T09:25:14Z","timestamp":1423214714000},"page":"67-72","source":"Crossref","is-referenced-by-count":7,"title":["AMP promotes oxygen consumption and ATP synthesis in heart mitochondria through the adenylate kinase reaction: an NMR spectroscopy and polarography study"],"prefix":"10.1002","volume":"33","author":[{"given":"Nicolai M.","family":"Doliba","sequence":"first","affiliation":[{"name":"University of Pennsylvania  Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andriy M.","family":"Babsky","sequence":"additional","affiliation":[{"name":"University of Pennsylvania  Philadelphia PA USA"},{"name":"Ivan Franko National University of Lviv  Lviv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nataliya M.","family":"Doliba","sequence":"additional","affiliation":[{"name":"Actelion Clinical Research  Cherry Hill NJ USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzanne L.","family":"Wehrli","sequence":"additional","affiliation":[{"name":"Children's Hospital of Philadelphia  Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary D.","family":"Osbakken","sequence":"additional","affiliation":[{"name":"Osbakken Consulting, LLC, and Visiting Research Professor, School of Biomedical Engineering, Science and Health Systems Drexel University  Philadelphia PA 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