{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T05:19:48Z","timestamp":1788412788319,"version":"build-2803163510"},"reference-count":20,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,5,22]],"date-time":"2015-05-22T00:00:00Z","timestamp":1432252800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:p>Increased tight junction (TJ) barrier permeability, induced by tumour necrosis factor (TNF)\u2010<jats:italic>\u03b1<\/jats:italic>, may lead to the defects in TJ barrier and subsequent development of inflammation. Recent evidence suggests that miR\u201021 is implicated in inflammatory diseases. However, the physiological role of miR\u201021 in intestinal permeability remains elusive. This study aimed to explore the role of miR\u201021 in intestinal epithelial tight junction permeability. The filter\u2010grown Caco\u20102 monolayers model system was established to mimic intestinal barrier defect. The tight junction proteins were detected by immunofluorescence and western blot analysis. The expression of miR\u201021 was assessed by real\u2010time polymerase chain reaction (PCR). We found that the expression of miR\u201021 was increased significantly in TNF\u2010<jats:italic>\u03b1<\/jats:italic> induced intestinal TJ barrier defect model. miR\u201021 overexpression significantly enhanced while miR\u201021 knockdown significantly decreased intestinal permeability. In addition, miR\u201021 overexpression significantly increased while miR\u201021 knockdown significantly decreased the levels of interleukin\u20106, interleukin\u20108 and prostaglandin E2 in cell culture medium. Furthermore, miR\u201021 positively regulated Akt phosphorylation and negatively regulated Phosphatase and tensin homolog (PTEN) expression in Caco\u20102 cells. Our results suggest that miR\u201021 may regulate intestinal epithelial tight junction permeability through PTEN\/PI3K\/Akt signalling pathway. This promotes the feasibility of targeting miR\u201021 in the clinical to preserve the intestinal barrier. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/cbf.3109","type":"journal-article","created":{"date-parts":[[2015,5,22]],"date-time":"2015-05-22T05:58:29Z","timestamp":1432274309000},"page":"235-240","source":"Crossref","is-referenced-by-count":81,"title":["MicroRNA\u201021 regulates intestinal epithelial tight junction permeability"],"prefix":"10.1002","volume":"33","author":[{"given":"Lin","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Gastroenterology Jinshan Hospital of Fudan University  Shanghai Jinshan China"},{"name":"Center of Emergency and Intensive Care Unit Jinshan Hospital, Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Shen","sequence":"additional","affiliation":[{"name":"Center of Emergency and Intensive Care Unit Jinshan Hospital, Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Jinshan Hospital of Fudan University  Shanghai Jinshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoming","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Jinshan Hospital of Fudan University  Shanghai Jinshan China"},{"name":"Departments of Internal Medicine, Shanghai Medical College Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,5,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2008.150888"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(93)90882-H"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/003655200750056637"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199710093371502"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa050516"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2002.31874"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00173.2003"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2007.02.052"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI24970"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(03)01057-6"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2011.07.005"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2013.03.122"},{"key":"e_1_2_7_14_1","first-page":"590","article-title":"Autoradiographic determination of permeation pathway of permeability probes across intestinal and tracheal epithelia","volume":"122","author":"Ma TY","year":"1993","journal-title":"J Lab Clin Med"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1840"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.seminoncol.2011.08.009"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2008.00556.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2008.07.068"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-10-2579"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-03-081133"},{"key":"e_1_2_7_21_1","article-title":"MiR\u201021 induced angiogenesis through AKT and ERK activation and HIF\u20101\u03b1 expression","volume":"6","author":"Liu LZ","year":"2011","journal-title":"PLoS One"}],"container-title":["Cell Biochemistry and Function"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcbf.3109","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbf.3109","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:49:01Z","timestamp":1760057341000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/cbf.3109"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5,22]]},"references-count":20,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2015,6]]}},"alternative-id":["10.1002\/cbf.3109"],"URL":"https:\/\/doi.org\/10.1002\/cbf.3109","archive":["Portico"],"relation":{},"ISSN":["0263-6484","1099-0844"],"issn-type":[{"value":"0263-6484","type":"print"},{"value":"1099-0844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,22]]}}}