{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:37:15Z","timestamp":1786977435318,"version":"build-2736575974"},"reference-count":54,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T00:00:00Z","timestamp":1436832000000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["R01EY023568"],"award-info":[{"award-number":["R01EY023568"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["R01EY020886"],"award-info":[{"award-number":["R01EY020886"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["T32EY023202"],"award-info":[{"award-number":["T32EY023202"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["P30EY021725"],"award-info":[{"award-number":["P30EY021725"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008126","name":"Dana-Farber\/Harvard Cancer Center","doi-asserted-by":"publisher","award":["P30CA006516"],"award-info":[{"award-number":["P30CA006516"]}],"id":[{"id":"10.13039\/100008126","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["P01CA120964"],"award-info":[{"award-number":["P01CA120964"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:title>\u00a0<\/jats:title>\n                  <jats:p>\n                    Corneal scarring is the result of a disease, infection or injury. The resulting scars cause significant loss of vision or even blindness. To\u2010date, the most successful treatment is corneal transplantation, but it does not come without side effects. One of the corneal dystrophies that are correlated with corneal scarring is keratoconus (KC). The onset of the disease is still unknown; however, altered cellular metabolism has been linked to promoting the fibrotic phenotype and therefore scarring. We have previously shown that human keratoconus cells (HKCs) have altered metabolic activity when compared to normal human corneal fibroblasts (HCFs). In our current study, we present evidence that quercetin, a natural flavonoid, is a strong candidate for regulating metabolic activity of both HCFs and HKCs\n                    <jats:italic>in vitro<\/jats:italic>\n                    and therefore a potential therapeutic to target the altered cellular metabolism characteristic of HKCs. Targeted mass spectrometry\u2010based metabolomics was performed on HCFs and HKCs with and without quercetin treatment in order to identify variations in metabolite flux. Overall, our study reveals a novel therapeutic target OF Quercetin on corneal stromal cell metabolism in both healthy and diseased states. Clearly, further studies are necessary in order to dissect the mechanism of action of quercetin. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/cbf.3122","type":"journal-article","created":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T15:21:31Z","timestamp":1436887291000},"page":"341-350","source":"Crossref","is-referenced-by-count":19,"title":["Quercetin modulates keratoconus metabolism\n                    <i>in vitro<\/i>"],"prefix":"10.1002","volume":"33","author":[{"given":"Tina B","family":"McKay","sequence":"first","affiliation":[{"name":"Department of Cell Biology University of Oklahoma Health Sciences Center  Oklahoma City OK USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akhee","family":"Sarker\u2010Nag","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology\/Dean McGee Eye Institute University of Oklahoma Health Sciences Center  Oklahoma City OK USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Desiree'","family":"Lyon","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology\/Dean McGee Eye Institute University of Oklahoma Health Sciences Center  Oklahoma City OK USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John M","family":"Asara","sequence":"additional","affiliation":[{"name":"Division of Signal Transduction, Beth Israel Deaconess Medical Center and Department of Medicine Harvard Medical School  Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitrios","family":"Karamichos","sequence":"additional","affiliation":[{"name":"Department of Cell Biology University of Oklahoma Health Sciences Center  Oklahoma City OK USA"},{"name":"Department of Ophthalmology\/Dean McGee Eye Institute University of Oklahoma Health Sciences Center  Oklahoma City OK USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,7,14]]},"reference":[{"key":"e_1_2_7_2_1","volume-title":"Molecular Biology of the Cell","author":"Bea A","year":"2002"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj20120427"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1250256"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2013.04.005"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2014.12.007"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2013.01.019"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M111.014688"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2012.024"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1409844111"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2015.01.007"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep04608"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0074223"},{"key":"e_1_2_7_14_1","first-page":"1210","article-title":"Metabolomic analysis of human vitreous humor differentiates ocular inflammatory disease","volume":"15","author":"Young SP","year":"2009","journal-title":"Mol Vis"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1221969110"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcrs.2010.12.037"},{"key":"e_1_2_7_17_1","first-page":"231","article-title":"Development and roles of collagenous matrices in the embryonic avian cornea","volume":"17","author":"Linsenmayer TF","year":"1998","journal-title":"Prog Retin Eye Res"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2012.03.018"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2014.09.013"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-200011000-00009"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ico.0000164831.87593.08"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.3109\/02713683.2012.754904"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/bf00395419"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(00)86321-9"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(00)80748-7"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep09003"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/jfb3040760"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/term.429"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks374"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.971"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(78)90368-9"},{"key":"e_1_2_7_32_1","first-page":"449","article-title":"Glucose oxidation in the chick cornea: effect of diamide on the pentose shunt","volume":"17","author":"Masterson E","year":"1978","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4835(84)90048-4"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4835(91)90135-2"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ge.20.120186.002243"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/138.10.2021S"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2840001"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmbbm.2010.09.014"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2014.02.007"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.11-9305"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.TEA.2013.0167"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.04-0288"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1207\/s15327914nc5401_3"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.2174\/09298673113209990120"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1021\/np300670w"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2011.04.023"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi025952r"},{"key":"e_1_2_7_48_1","first-page":"1177","article-title":"The antiproliferative effect of quercetin in cancer cells is mediated via inhibition of the PI3K\u2013Akt\/PKB pathway","volume":"26","author":"Gulati N","year":"2006","journal-title":"Anticancer Res"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2012.02.017"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1158\/1940-6207.capr-09-0220"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.07-0477"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2010.03.016"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.04-1225"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.06-0635"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.3109\/02713689109013850"}],"container-title":["Cell Biochemistry and Function"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcbf.3122","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbf.3122","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:27:17Z","timestamp":1760056037000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/cbf.3122"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,7]]},"references-count":54,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2015,7]]}},"alternative-id":["10.1002\/cbf.3122"],"URL":"https:\/\/doi.org\/10.1002\/cbf.3122","archive":["Portico"],"relation":{},"ISSN":["0263-6484","1099-0844"],"issn-type":[{"value":"0263-6484","type":"print"},{"value":"1099-0844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,7]]}}}