{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T07:23:26Z","timestamp":1790061806735,"version":"4.0.1"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2015,11,3]],"date-time":"2015-11-03T00:00:00Z","timestamp":1446508800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Scientific Research Funding of Education Department of Heilongjiang Province, China","award":["12541336"],"award-info":[{"award-number":["12541336"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:p>G protein\u2010coupled estrogen receptor (GPER) is identified as a critical estrogen receptor, in addition to the classical estrogen receptors ER<jats:italic>\u03b1<\/jats:italic> and ER<jats:italic>\u03b2<\/jats:italic>. In ER<jats:italic>\u03b1<\/jats:italic>\u2010negative ovarian cancer cells, our previous studies have found that estrogen stimulated cell proliferation and metastasis via GPER. However, the ligand\u2010independent function of GPER in ovarian cancer cells is still not clear. Herein, we describe that GPER has a co\u2010expression with ER<jats:italic>\u03b1<\/jats:italic> and ER<jats:italic>\u03b2<\/jats:italic>, which are first determined in SKOV3 ovarian cancer cell line. In the absence of estrogen, GPER depletion by specific siRNA inhibits the proliferation, migration and invasion of SKOV3 cells. Whereas abrogation of ER<jats:italic>\u03b1<\/jats:italic> or ER<jats:italic>\u03b2<\/jats:italic> by specific antagonist MPP and PHTPP has the opposite effects for stimulation of cell growth. Markedly, GPER knockdown attenuates MPP or PHTPP\u2010induced cell proliferation, migration and invasion. Furthermore, GPER modulates protein expression of the cell cycle critical components, c\u2010fos and cyclin D1 and factors for cancer cell invasion and metastasis, matrix metalloproteinase 2 (MMP\u20102) and MMP\u20109. These findings establish that GPER ligand\u2010independently stimulates the proliferation, migration and invasion of SKOV3 cells. Knockdown of GPER attenuates the progression of ovarian cancer that caused by functional loss of ER<jats:italic>\u03b1<\/jats:italic> or ER<jats:italic>\u03b2<\/jats:italic>. Targeting GPER provides new aspect as a potential therapeutic strategy in ovarian cancer. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/cbf.3154","type":"journal-article","created":{"date-parts":[[2015,11,3]],"date-time":"2015-11-03T00:59:58Z","timestamp":1446512398000},"page":"552-559","source":"Crossref","is-referenced-by-count":40,"title":["Role of GPER on proliferation, migration and invasion in ligand\u2010independent manner in human ovarian cancer cell line SKOV3"],"prefix":"10.1002","volume":"33","author":[{"given":"Yan","family":"Yan","sequence":"first","affiliation":[{"name":"Laboratory of Reproductive Endocrinology, Department of Physiology Harbin Medical University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueli","family":"Jiang","sequence":"additional","affiliation":[{"name":"Laboratory of Reproductive Endocrinology, Department of Physiology Harbin Medical University  Harbin 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