{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T22:11:28Z","timestamp":1784585488791,"version":"3.55.0"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,3,9]],"date-time":"2016-03-09T00:00:00Z","timestamp":1457481600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:p>The molecular signalling of placental growth factor (PlGF), a member of the vascular endothelial growth factor family, was not uncovered in human adenocarcinoma gastric cell line (AGS). The purpose of this study was to examine the inhibitory effects of PlGF knockdown on cell proliferation, apoptosis and migration through p38 mitogen\u2010activated protein kinase (p38MAPK) and PI3K pathways in human adenocarcinoma gastric cell line (AGS). To study PlGF knockdown effect, AGS cells were treated with 40\u2009pmol of small interfering RNA (siRNA) related to <jats:italic>PlGF<\/jats:italic> gene and also a scrambled siRNA as control. Trypan Blue and Anexin V staining of AGS cells treated with <jats:italic>PlGF<\/jats:italic>\u2010specific siRNA showed induction of apoptosis. Wound healing assay and zymography indicated that cellular migration and matrix metalloproteinases activities were reduced in response to PlGF knockdown. Phosphorylation of Akt and p38MAPK was reduced in AGS cells treated with <jats:italic>PlGF<\/jats:italic>\u2010specific siRNA. PlGF knockdown decreased transcripts of <jats:italic>PI3K<\/jats:italic>, <jats:italic>Akt<\/jats:italic>, <jats:italic>p38MAPK<\/jats:italic>, <jats:italic>PCNA<\/jats:italic>, <jats:italic>Caspase\u20103<\/jats:italic>, <jats:italic>OCT3<\/jats:italic>\/<jats:italic>OCT4<\/jats:italic> and <jats:italic>CD44<\/jats:italic>, but elevated <jats:italic>p53<\/jats:italic> and <jats:italic>SOX2<\/jats:italic> transcripts. Our results indicated that PlGF knockdown decreased migration and induced apoptosis through PI3K\/Akt1 and p38MAPK signal transduction in AGS cells. Copyright \u00a9 2016 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/cbf.3176","type":"journal-article","created":{"date-parts":[[2016,3,12]],"date-time":"2016-03-12T02:02:57Z","timestamp":1457748177000},"page":"173-180","source":"Crossref","is-referenced-by-count":34,"title":["PlGF knockdown inhibited tumor survival and migration in gastric cancer cell via PI3K\/Akt and p38MAPK pathways"],"prefix":"10.1002","volume":"34","author":[{"given":"Hassan","family":"Akrami","sequence":"first","affiliation":[{"name":"Department of Biology, Faculty of Science Razi University  Kermanshah Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatemeh","family":"Mahmoodi","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science Razi University  Kermanshah Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Somaye","family":"Havasi","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science Razi 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