{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T13:47:46Z","timestamp":1767707266813,"version":"build-2065373602"},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,2,23]],"date-time":"2017-02-23T00:00:00Z","timestamp":1487808000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL110740","HL107957"],"award-info":[{"award-number":["HL110740","HL107957"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:p>It has been reported that CXCR4\u2010overexpressing mesenchymal stem cells (MSC<jats:sup>CX4<\/jats:sup>) can repair heart tissue post myocardial infarction. This study aims to investigate the MSCCX4\u2010derived paracrine cardio\u2010protective signaling in the presence of myocardial infarction. Mesenchymal stem cells (MSCs) were divided into 3 groups: MSC only, MSC<jats:sup>CX4<\/jats:sup>, and <jats:italic>CXCR4<\/jats:italic> gene\u2010specific siRNA\u2010transduced MSC. Mesenchymal stem cells were exposed to hypoxia, and then MSCs\u2010conditioned culture medium was incubated with neonatal and adult cardiomyocytes, respectively. Cell proliferation\u2013regulating genes were assessed by real\u2010time polymerase chain reaction (RT\u2010PCR). In vitro: The number of cardiomyocytes undergoing DNA synthesis, cytokinesis, and mitosis was increased to a greater extent in MSC<jats:sup>CX4<\/jats:sup> medium\u2010treated group than control group, while this proproliferative effect was reduced in <jats:italic>CXCR4<\/jats:italic> gene\u2010specific siRNA\u2010transduced MSC\u2013treated cells. Accordingly, the maximal enhancement of vascular endothelial growth factor, cyclin 2, and transforming growth factor\u2010\u03b22 was observed in hypoxia\u2010exposed MSC<jats:sup>CX4<\/jats:sup>. In vivo: MSCs were labeled with enhanced green fluorescent protein (EGFP) and engrafted into injured myocardium in rats. The number of EGFP and CD31 positive cells in the MSC<jats:sup>CX4<\/jats:sup> group was significantly increased than other 2 groups, associated with the reduced left ventricular (LV) fibrosis, the increased LV free wall thickness, the enhanced angiogenesis, and the improved contractile function. CXCR4 overexpression can mobilize MSCs into ischemic area, whereby these cells can promoted angiogenesis and alleviate LV remodeling via paracrine signaling mechanism.<\/jats:p>","DOI":"10.1002\/cbf.3254","type":"journal-article","created":{"date-parts":[[2017,2,23]],"date-time":"2017-02-23T23:03:43Z","timestamp":1487891023000},"page":"113-123","source":"Crossref","is-referenced-by-count":30,"title":["Paracrine effect of CXCR4\u2010overexpressing mesenchymal stem cells on ischemic heart injury"],"prefix":"10.1002","volume":"35","author":[{"given":"Shi\u2010Zheng","family":"Wu","sequence":"first","affiliation":[{"name":"Qinghai Provincial People's Hospital  Xining Qinghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying\u2010Lan","family":"Li","sequence":"additional","affiliation":[{"name":"Qinghai Provincial People's Hospital  Xining Qinghai China"},{"name":"Research Center for High Altitude Medicine Medical College of Qinghai University  Xining Qinghai China"},{"name":"Department of Pathology and Laboratory Medicine, College of Medicine University of Cincinnati Medical Center  Cincinnati Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, College of Medicine University of Cincinnati Medical Center  Cincinnati Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1499-4347","authenticated-orcid":false,"given":"Wen\u2010Feng","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, College of Medicine University of Cincinnati Medical Center  Cincinnati Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jialiang","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, College of Medicine University of Cincinnati Medical Center  Cincinnati Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Paul","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, College of Medicine University of Cincinnati Medical Center  Cincinnati Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, College of Medicine University of Cincinnati Medical Center  Cincinnati Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi\u2010Chao","family":"Wu","sequence":"additional","affiliation":[{"name":"Guangdong Cardiovascular Institute Guangdong General Hospital  Guangzhou China"},{"name":"Department of Pathology and Laboratory Medicine, College of Medicine University of Cincinnati Medical Center  Cincinnati Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meifeng","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, College of Medicine University of Cincinnati Medical Center  Cincinnati Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Zhu","sequence":"additional","affiliation":[{"name":"Guangdong Cardiovascular Institute Guangdong General Hospital  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yigang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, College of Medicine University of Cincinnati Medical Center  Cincinnati Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,2,23]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.107.5.1911"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.7.23.7239"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1619"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0607382103"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000049105.15329.1C"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.107.153544"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E06\u201012\u20101064"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2004\u20100342"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1042\/BC20040069"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1527-3466.2005.tb00173.x"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2009.10.032"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2001.280.2.H899"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000020201.44772.67"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2007.11.011"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvm025"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01163.2006"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2004.09.004"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2004.10.022"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.04.098"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4644(199702)64:2<278::AID-JCB11>3.0.CO;2-F"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00013.2005"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm912"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.5966\/sctm.2015\u20100032"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2006.06.074"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000218272.18669.6e"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2006.02.004"}],"container-title":["Cell Biochemistry and Function"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcbf.3254","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbf.3254","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:56:09Z","timestamp":1760057769000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/cbf.3254"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2,23]]},"references-count":26,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,3]]}},"alternative-id":["10.1002\/cbf.3254"],"URL":"https:\/\/doi.org\/10.1002\/cbf.3254","archive":["Portico"],"relation":{},"ISSN":["0263-6484","1099-0844"],"issn-type":[{"type":"print","value":"0263-6484"},{"type":"electronic","value":"1099-0844"}],"subject":[],"published":{"date-parts":[[2017,2,23]]}}}