{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T11:59:32Z","timestamp":1782215972489,"version":"3.54.5"},"reference-count":46,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,2,27]],"date-time":"2019-02-27T00:00:00Z","timestamp":1551225600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100008386","name":"Marmara \u00dcniversitesi","doi-asserted-by":"publisher","award":["SAG\u2010C\u2010DRP\u2010080415\u20100099"],"award-info":[{"award-number":["SAG\u2010C\u2010DRP\u2010080415\u20100099"]}],"id":[{"id":"10.13039\/501100008386","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:sec><jats:label\/><jats:p>The anti\u2010catabolic bisphosphonate alendronate is considered as the first\u2010line medical treatment in post\u2010menopausal osteoporosis; but several side effects, including gastric mucosal injury, are associated with its use. The aim was to elucidate whether combined treatment with melatonin plus alendronate would be more advantageous in the maintenance of bone and the prevention of gastric side effects. Under anaesthesia, female Sprague\u2010Dawley rats underwent bilateral ovariectomy (OVX), while control group had sham surgery. Four weeks after the surgery, OVX rats were treated with saline, melatonin (25\u00a0\u03bcg\/mL\/d), alendronate (70\u00a0\u03bcg\/kg\/wk), melatonin\u00a0+\u00a0alendronate, melatonin\u00a0+\u00a0melatonin receptor antagonist (luzindole, 10\u00a0\u03bcg\/kg\/d) or alendronate\u00a0+\u00a0melatonin\u00a0+\u00a0luzindole for 8\u00a0weeks. Rats were euthanized at the end of 12th week. Runx2 expression, apoptotic cells, and trabecular thickness were evaluated in tibiae, while gastric tissues were analysed for oxidative injury parameters. In all OVX groups, Runx2 expression was depressed. Saline\u2010treated OVX group presented an extreme decrease in calcified area in opposition to melatonin\u2010 or alendronate\u2010treated groups, while the bones in alendronate\u00a0+\u00a0melatonin\u2010treated group were similar to those of the sham\u2010operated group. Concomitant with the improvements examined histologically in bone tissues, quantitative TUNEL (+) cells were similarly lower in alendronate\u2010 or melatonin\u2010treated groups. Oxidative gastric damage was increased in saline\u2010 or alendronate\u2010treated groups, which were depressed in the presence of melatonin. Although melatonin and alendronate exerted similar supportive effects on the maintenance of bone mass, melatonin may have a more advantageous impact by protecting against OVX\u2010induced gastric injury, which was aggravated by alendronate use.<\/jats:p><\/jats:sec><jats:sec><jats:title>Highlights<\/jats:title><jats:p>Our results demonstrate that alendronate and melatonin had similar supportive effects on the maintenance of bone mass, while melatonin prevented the gastric side effects of alendronate, making this combination an advisable therapeutic approach in the treatment of menopausal osteoporosis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbf.3379","type":"journal-article","created":{"date-parts":[[2019,2,28]],"date-time":"2019-02-28T01:11:45Z","timestamp":1551316305000},"page":"102-112","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Melatonin supports alendronate in preserving bone matrix and prevents gastric inflammation in ovariectomized rats"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8259-521X","authenticated-orcid":false,"given":"Esra Bihter","family":"G\u00fcrler","sequence":"first","affiliation":[{"name":"School of Medicine, Physiology Department Marmara University, Istanbul  Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00d6zlem Tu\u011f\u00e7e","family":"\u00c7ilingir\u2010Kaya","sequence":"additional","affiliation":[{"name":"School of Medicine Histology &amp; Embryology Department, Marmara University, Istanbul, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irem","family":"Peker Ey\u00fcboglu","sequence":"additional","affiliation":[{"name":"School of Medicine Medical Biology Department, Marmara University, Istanbul  Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feriha","family":"Ercan","sequence":"additional","affiliation":[{"name":"School of Medicine Histology 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