{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T17:30:06Z","timestamp":1771263006429,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,10,31]],"date-time":"2019-10-31T00:00:00Z","timestamp":1572480000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"College of Life Sciences, University of Chinese Academy of Sciences","award":["KJRH2015-006"],"award-info":[{"award-number":["KJRH2015-006"]}]},{"name":"Knowledge Innovation Program of The Chinese Academy of Sciences","award":["KSCX2-EW-J-29"],"award-info":[{"award-number":["KSCX2-EW-J-29"]}]},{"name":"Knowledge Innovation Program of The Chinese Academy of Sciences","award":["Y129015EA2"],"award-info":[{"award-number":["Y129015EA2"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:sec><jats:label\/><jats:p>Uncarboxylated osteocalcin (unOc) is an osteoblast\u2010derived hormone with multiple regulatory functions. Osteocalcin knockdown delays the maturation of mineral species and downregulates the expression of osteogenic\u2010specific genes in human mesenchymal stromal cells. However, the underlying mechanisms remain unclear. Here, we investigated the effects of unOc on the osteogenic differentiation of mouse bone marrow\u2013derived mesenchymal stem cells (BMSCs) and discovered that unOc promoted osteogenic differentiation of BMSCs, which was characterized by increases in alkaline phosphatase (ALP) activity, type I collagen (COLI) production, calcified nodule formation, and expression of osteogenic\u2010specific genes including the osterix, runt\u2010related transcription factor 2 (Runx2), ALP, and COLI genes. Further experiments indicated that unOc promoted the osteogenic differentiation of BMSCs via activation of the Erk\u2010Smad\/\u03b2\u2010catenin signalling pathways.<\/jats:p><\/jats:sec><jats:sec><jats:title>Significance of the study<\/jats:title><jats:p>Osteoporosis is associated with the osteogenic differentiation of BMSCs. In recent years, the role of unOc function as an endocrine hormone has received much attention. In this study, we reported for the first time that unOc promoted the osteogenic differentiation of mouse BMSCs through Erk\u2010Smad\/\u03b2\u2010catenin signalling pathway. Our results highlight the importance of unOc as a hormone in promoting the osteogenic differentiation of BMSCs, indicating that this hormone may be beneficial in treatments for osteoporosis and fracture healing.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbf.3457","type":"journal-article","created":{"date-parts":[[2019,11,1]],"date-time":"2019-11-01T01:05:30Z","timestamp":1572570330000},"page":"87-96","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Uncarboxylated osteocalcin promotes osteogenic differentiation of mouse bone marrow\u2013derived mesenchymal stem cells by activating the Erk\u2010Smad\/\u03b2\u2010catenin signalling pathways"],"prefix":"10.1002","volume":"38","author":[{"given":"Zhongsheng","family":"Liu","sequence":"first","affiliation":[{"name":"Medical School University of Chinese Academy of Sciences  Beijing 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