{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T16:28:52Z","timestamp":1790267332888,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,12,30]],"date-time":"2019-12-30T00:00:00Z","timestamp":1577664000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan","award":["18K09509"],"award-info":[{"award-number":["18K09509"]}]},{"name":"Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan","award":["26293396"],"award-info":[{"award-number":["26293396"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:sec><jats:label\/><jats:p>Podosome formation in osteoclasts is an important initial step in osteoclastic bone resorption. Mice lacking c\u2010Src (c\u2010Src<jats:sup>\u2212\/\u2212<\/jats:sup>) exhibited osteopetrosis due to a lack of podosome formation in osteoclasts. We previously identified p130Cas (Crk\u2010associated substrate [Cas]) as one of c\u2010Src downstream molecule and osteoclast\u2010specific p130Cas\u2010deficient (p130Cas<jats:sup>\u0394OCL\u2212\/\u2212<\/jats:sup>) mice also exhibited a similar phenotype to c\u2010Src<jats:sup>\u2212\/\u2212<\/jats:sup> mice, indicating that the c\u2010Src\/p130Cas plays an important role for bone resorption by osteoclasts. In this study, we performed a cDNA microarray and compared the gene profiles of osteoclasts from c\u2010Src<jats:sup>\u2212\/\u2212<\/jats:sup> or p130Cas<jats:sup>\u0394OCL\u2212\/\u2212<\/jats:sup> mice with wild\u2010type (WT) osteoclasts to identify downstream molecules of c\u2010Src\/p130Cas involved in bone resorption. Among several genes that were commonly downregulated in both c\u2010Src<jats:sup>\u2212\/\u2212<\/jats:sup> and p130Cas<jats:sup>\u0394OCL\u2212\/\u2212<\/jats:sup> osteoclasts, we identified kinesin family protein 1c (Kif1c), which regulates the cytoskeletal organization. Reduced Kif1c expression was observed in both c\u2010Src<jats:sup>\u2212\/\u2212<\/jats:sup> and p130Cas<jats:sup>\u0394OCL\u2212\/\u2212<\/jats:sup> osteoclasts compared with WT osteoclasts. Kif1c exhibited a broad tissue distribution, including osteoclasts. Knockdown of Kif1c expression using shRNAs in WT osteoclasts suppressed actin ring formation. Kif1c overexpression restored bone resorption subsequent to actin ring formation in p130Cas<jats:sup>\u0394OCL\u2212\/\u2212<\/jats:sup> osteoclasts but not c\u2010Src<jats:sup>\u2212\/\u2212<\/jats:sup> osteoclasts, suggesting that Kif1c regulates osteoclastic bone resorption in the downstream of p130Cas (191 words).<\/jats:p><\/jats:sec><jats:sec><jats:title>Significance of the study<\/jats:title><jats:p>We previously showed that the c\u2010Src\/p130Cas (Cas) plays an important role for bone resorption by osteoclasts. In this study, we identified kinesin family protein 1c (Kif1c), which regulates the cytoskeletal organization, as a downstream molecule of c\u2010Src\/p130Cas axis, using cDNA microarray. Knockdown of Kif1c expression using shRNAs in wild\u2010type osteoclasts suppressed actin ring formation. Kif1c overexpression restored bone resorption subsequent to actin ring formation in osteoclast\u2010specific p130Cas\u2010deficient (p130Cas<jats:sup>\u0394OCL\u2212\/\u2212<\/jats:sup>) osteoclasts but not c\u2010Src<jats:sup>\u2212\/\u2212<\/jats:sup> osteoclasts, suggesting that Kif1c regulates osteoclastic bone resorption in the downstream of p130Cas.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbf.3476","type":"journal-article","created":{"date-parts":[[2019,12,30]],"date-time":"2019-12-30T19:33:23Z","timestamp":1577734403000},"page":"300-308","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Kif1c regulates osteoclastic bone resorption as a downstream molecule of p130Cas"],"prefix":"10.1002","volume":"38","author":[{"given":"Miki","family":"Kobayakawa","sequence":"first","affiliation":[{"name":"Division of Molecular Signaling and Biochemistry, Department of Health Improvement Kyushu Dental University  Kitakyushu Japan"},{"name":"Division of Oral Reconstruction and Rehabilitation, Department of Oral Functional Reconstruction Kyushu Dental University  Kitakyushu Japan"},{"name":"Laboratory of Molecular and Cellular Biochemistry Faculty of Dental Science, Kyushu University  Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8505-0431","authenticated-orcid":false,"given":"Takuma","family":"Matsubara","sequence":"additional","affiliation":[{"name":"Division of Molecular Signaling and Biochemistry, Department of Health Improvement Kyushu Dental University  Kitakyushu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akiko","family":"Mizokami","sequence":"additional","affiliation":[{"name":"Oral Health\/Brain Health\/Total Health Research Center, Faculty of Dental Science Kyushu University  Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumitaka","family":"Hiura","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular and Cellular Biochemistry Faculty of Dental Science, Kyushu University  Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nana","family":"Takakura","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular and Cellular Biochemistry Faculty of Dental Science, Kyushu University  Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoichiro","family":"Kokabu","sequence":"additional","affiliation":[{"name":"Division of Molecular Signaling and Biochemistry, Department of Health Improvement Kyushu Dental University  Kitakyushu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miho","family":"Matsuda","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular and Cellular Biochemistry Faculty of Dental Science, Kyushu University  Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisataka","family":"Yasuda","sequence":"additional","affiliation":[{"name":"Nagahama Institute for Biochemical Science Oriental Yeast Co., Ltd.  Shiga Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ichiro","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Faculty of Health and Medical Science Teikyo Heisei University 2\u201051\u20104 Higashi\u2010Ikebukuro  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yosuke","family":"Takei","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Neuroscience, Faculty of Medicine University of Tsukuba  Tsukuba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroaki","family":"Honda","sequence":"additional","affiliation":[{"name":"Field of Human Disease Models, Major in Advanced Life Sciences and Medicine, Institute of Laboratory Animals Tokyo Women's Medical University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryuji","family":"Hosokawa","sequence":"additional","affiliation":[{"name":"Division of Oral Reconstruction and Rehabilitation, Department of Oral Functional Reconstruction Kyushu Dental University  Kitakyushu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3616-5299","authenticated-orcid":false,"given":"Eijiro","family":"Jimi","sequence":"additional","affiliation":[{"name":"Division of Molecular Signaling and Biochemistry, Department of Health Improvement Kyushu Dental University  Kitakyushu Japan"},{"name":"Laboratory of Molecular and Cellular Biochemistry Faculty of Dental Science, Kyushu University  Fukuoka Japan"},{"name":"Oral Health\/Brain Health\/Total Health Research Center, Faculty of Dental Science Kyushu University  Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,12,30]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3109\/s10165-011-0530-8"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-018-1636-2"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2006.04.010"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90499-O"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116032"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.18.11144"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.1936"},{"issue":"4","key":"e_1_2_11_9_1","doi-asserted-by":"crossref","first-page":"e00425","DOI":"10.1128\/MCB.00425-17","article-title":"The Actin\u2010binding protein PPP1r18 regulates maturation, Actin organization, and bone resorption activity of osteoclasts","volume":"38","author":"Matsubara T","year":"2018","journal-title":"Mol Cell Biol"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.22.3.866-873.2002"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.017269"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.241"},{"issue":"3","key":"e_1_2_11_13_1","first-page":"19","article-title":"Signal transduction pathways of calcitonin\/calcitonin receptor regulating cytoskeletal organization and bone\u2010resorbing activity of osteoclasts","volume":"52","author":"Tanaka S","year":"2006","journal-title":"Cell Mol Biol. (Noisy\u2010le\u2010Grand)"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02425"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e09-03-0248"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00578-13"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E05-11-1010"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-12-257089"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E10-05-0394"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.1844"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2012.10.003"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.1725"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.149633"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.5.1649-1658.2000"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00517.2003"}],"container-title":["Cell Biochemistry and Function"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcbf.3476","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbf.3476","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbf.3476","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbf.3476","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T19:02:47Z","timestamp":1760036567000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/cbf.3476"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,30]]},"references-count":24,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,4]]}},"alternative-id":["10.1002\/cbf.3476"],"URL":"https:\/\/doi.org\/10.1002\/cbf.3476","archive":["Portico"],"relation":{},"ISSN":["0263-6484","1099-0844"],"issn-type":[{"value":"0263-6484","type":"print"},{"value":"1099-0844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12,30]]},"assertion":[{"value":"2019-05-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}