{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T10:35:28Z","timestamp":1762079728094,"version":"build-2065373602"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,12,29]],"date-time":"2019-12-29T00:00:00Z","timestamp":1577577600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Serbian Ministry of Education, Science and Technological Development","award":["OI175053"],"award-info":[{"award-number":["OI175053"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:sec><jats:label\/><jats:p>This study has been performed to determine the mechanism of activation of the myeloid related S100A proteins by inflammatory cytokines in myeloproliferative neoplasm (MPN). Besides microarray analysis of MPN\u2010derived CD34<jats:sup>+<\/jats:sup> cells, we analysed the pro\u2010inflammatory IL6 and anti\u2010inflammatory IL10 dependence of NF\u2010\u03baB, PI3K\u2010AKT, and JAK\u2010STAT signalling during induction of S100A proteins in mononuclear cells of MPN, by immunoblotting and flow cytometry. We observed the reduced gene expression linked to NF\u2010\u03baB and inflammation signalling in MPN\u2010derived CD34<jats:sup>+<\/jats:sup> cells. Both IL6 and IL10 reduced S100A8 and 100A9 protein levels mediated via NF\u2010\u03baB and PI3K signalling, respectively, in mononuclear cells of essential thrombocythemia (ET). We also determined the increased percentage of S100A8 and S100A9 positive granulocytes in ET and primary myelofibrosis, upgraded by the <jats:italic>JAK2<\/jats:italic>V617F mutant allele burden. S100A8\/9 heterodimer induced JAK1\/2\u2010dependent mitotic arrest of the ET\u2010derived granulocytes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Significance of the study<\/jats:title><jats:p>We demonstrated that inflammation reduced the myeloid related S100A8\/9 proteins by negative feedback mechanism in ET. S100A8\/9 can be a diagnostic marker of inflammation in MPN, supported by the concomitant NF\u2010\u03baB and JAK1\/2 signalling inhibition in regulation of myeloproliferation and therapy of MPN.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbf.3482","type":"journal-article","created":{"date-parts":[[2019,12,29]],"date-time":"2019-12-29T23:30:42Z","timestamp":1577662242000},"page":"362-372","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["IL6 inhibition of inflammatory S100A8\/9 proteins is NF\u2010\u03baB mediated in essential thrombocythemia"],"prefix":"10.1002","volume":"38","author":[{"given":"Milo\u0161","family":"Dikli\u0107","sequence":"first","affiliation":[{"name":"Department of Molecular Oncology, Institute for Medical Research University of Belgrade  Belgade 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