{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T20:05:18Z","timestamp":1774382718128,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,4,1]],"date-time":"2020-04-01T00:00:00Z","timestamp":1585699200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Research fund of Fujian Medical University","award":["2016QH074"],"award-info":[{"award-number":["2016QH074"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:sec><jats:label\/><jats:p>Invasion of dentinal tubules and pulp tissue by pathogenic bacteria may cause infection leading to pulpitis. Sirtuin 6 (SIRT6) is a NAD\u2010dependent protein deacetylase encoded by the SIRT6 gene. The effect of SIRT6 on lipopolysaccharide (LPS)\u2010induced pulpitis and its mechanism of action were discussed in this study. Dental pulp cells (DPCs) were extracted from human teeth and injected with LPS to induce inflammation. The cells injected with LPS showed substantially decreased expression of SIRT6. The overexpression of SIRT6, induced by plasmid\u2010transfection of DPCs with SIRT6 overexpressing vector, led to a marked decrease in proinflammatory cytokines (IL\u20106, IL\u20101\u03b2, and TNF\u2010\u03b1) and deactivation of NF kappa B pathway. Additionally, dentin matrix protein\u20101 (DMP1), a promoter of inflammation in dental pulp tissues, was downregulated. Further investigation revealed that SIRT6 promotes ubiquitination of the transient receptor potential vanilloid 1 (TRPV1) channel, leading to its degradation and deactivation. The role of TRPV1 in the anti\u2010inflammatory effects of SIRT6 was determined through incubation of SIRT6\u2010expressing dental pulp stem cells (DPSCs) with capsaicin. This incubation counteracted the effect of SIRT6 on cytokines and DMP1. The injection of lentivirus\u2010SIRT6 attenuated LPS\u2010induced pulpitis in vivo by suppressing TRPV1 activity. Thus, SIRT6 inhibits the TRPV1 channel during LPS\u2010induced inflammation of dental pulp.<\/jats:p><\/jats:sec><jats:sec><jats:title>Significance of the Study<\/jats:title><jats:p>This study discussed the effect of sirtuin 6 (SIRT6) on lipopolysaccharide (LPS)\u2010induced pulpitis as well as its mechanism of action and found that SIRT6 may be a negative regulator of pulpitis. Additionally, low expression of SIRT6 and high expression of transient receptor potential vanilloid 1 (TRPV1) in LPS\u2010treated human dental pulp cells are closely associated with proinflammatory cytokines, dentin matrix protein 1 expression, and activation of the NF\u2010\u03baB pathway, which indicated that TRPV1 may be a biomarker for pulpitis and the SIRT6\u2010TRPV1\u2010CGRP axis maybe a clinical target due to their role regulating inflammation and neuropathic pain.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbf.3528","type":"journal-article","created":{"date-parts":[[2020,4,1]],"date-time":"2020-04-01T03:31:11Z","timestamp":1585711871000},"page":"676-682","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Robust expression of <scp>SIRT6<\/scp> inhibits pulpitis via activation of the <scp>TRPV1<\/scp> channel"],"prefix":"10.1002","volume":"38","author":[{"given":"Jia","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Endodontics Affiliated Stomatological Hospital of Fujian Medical University  Fuzhou Fujian China"},{"name":"Fujian Provincial Key Laboratory of Stomatology  Fuzhou Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiran","family":"Chen","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Stomatology  Fuzhou Fujian China"},{"name":"Affiliated Stomatological Hospital of Fujian Medical University  Fuzhou Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zailing","family":"Qiu","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Stomatology  Fuzhou Fujian China"},{"name":"Affiliated Stomatological Hospital of Fujian Medical University  Fuzhou Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8273-5427","authenticated-orcid":false,"given":"Hongbing","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Endodontics Affiliated Stomatological Hospital of Fujian Medical University  Fuzhou Fujian China"},{"name":"Fujian Provincial Key Laboratory of Stomatology  Fuzhou Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,4]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2012.10.031"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0052523"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2010.12.012"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2009.07.005"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08197"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pathol.4.110807.092250"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.5395\/rde.2015.40.3.223"},{"key":"e_1_2_9_9_1","first-page":"647","article-title":"Inhibition of Sirtuin 6 induces Neuroblastoma differentiation","volume":"38","author":"Song HY","year":"2018","journal-title":"Anticancer Res"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2011.07.006"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.12396"},{"key":"e_1_2_9_12_1","first-page":"72","article-title":"Protective effect of SIRT6 against LPS\u2010induced human dental pulp cell apoptosis via regulating Ku70 deacetylation","volume":"10","author":"Feng Q","year":"2017","journal-title":"Int J Clin Exp Pathol"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2012.982"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2011.10.005"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910508401105"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2016.10.001"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1177\/1744806916661725"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034511413284"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2007.07.001"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-032713-120156"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.25704"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.109.001263"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5403-05.2006"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.288.5464.306"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.14-268300"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.03.051"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2016.11.007"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.5414\/CP201890"}],"container-title":["Cell Biochemistry and Function"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcbf.3528","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbf.3528","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbf.3528","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbf.3528","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T23:43:53Z","timestamp":1760053433000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/cbf.3528"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4]]},"references-count":27,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,7]]}},"alternative-id":["10.1002\/cbf.3528"],"URL":"https:\/\/doi.org\/10.1002\/cbf.3528","archive":["Portico"],"relation":{},"ISSN":["0263-6484","1099-0844"],"issn-type":[{"value":"0263-6484","type":"print"},{"value":"1099-0844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4]]},"assertion":[{"value":"2019-12-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-04-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}