{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T04:09:26Z","timestamp":1777435766682,"version":"3.51.4"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,5,20]],"date-time":"2020-05-20T00:00:00Z","timestamp":1589932800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"This work was supported by high-level hospital foster grants from Fujian Provincial Hospital, Fujian province, China","award":["2019HSJJ0"],"award-info":[{"award-number":["2019HSJJ0"]}]},{"name":"Joint Funds for the Innovation of Science and Technology, Fujian Province","award":["2017Y9068"],"award-info":[{"award-number":["2017Y9068"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:sec><jats:label\/><jats:p>The current study investigated the role of mesenchymal stem cells (MSCs) in repairing senile bone marrow injury and the underlying mechanism. Adenoviral vectors expressing green fluorescent protein (GFP) were used to label MSCs. The level of malondialdehyde (MDA) and activity of superoxide dismutase (SOD) were detected by thiobarbituric acid (TBA) and xanthine oxidation (XTO) methods. The proportions of CD34, CD3<jats:sup>+<\/jats:sup> cells, cell proliferation and apoptosis were determined by flow cytometry, Cell counting kit (CCK)\u20108 and comet assay. Tissues were stained by haematoxylin\u2013eosin (HE) staining and their changes were observed under a transmission electron microscopy. Expression levels of age\u2010related and autophagy\u2010related genes were detected by RT\u2010qPCR and Western Blot. MSCs were successfully implanted into the bone marrow of aging rats. We found that the SOD activity was increased and MDA content was reduced in MSCs group. The proportions of CD34 cells were significantly more in the MSCs group than those in the Model group, and bone marrow cell colony formation and cell viability were both greatly increased in MSCs group. The proportions of CD3<jats:sup>+<\/jats:sup> cells and level of Vascular endothelial growth factor (VEGF) were increased significantly, while IL\u20106 level was reduced greatly in MSCs group. Moreover, the bone marrow tissues of the model group were severely damaged, but those of the MSCs group were significantly improved. In addition, MSCs were involved in regulation of aging\u2010related genes and autophagy\u2010related genes. In conclusion, our findings showed that MSCs can repair bone marrow damage in aging rats, and regulate aging\u2010 and autophagy\u2010related genes and immune response.<\/jats:p><\/jats:sec><jats:sec><jats:title>Significance<\/jats:title><jats:p>This study investigated the role of MSCs in the repair of senile bone marrow injury and the underlying mechanism. The effects of MSCs on physiological and biochemical indicators, cell function, tissue structure differences and pathological changes in aging rats were studied. It was found that MSCs can repair bone marrow damage in aging rats. MSCs regulate aging and autophagy\u2010related genes and its involvement in immune response. Our findings improve the understandings on the regulatory mechanism of MSCs and provide key evidence for the study of MSCs in bone marrow repair.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbf.3548","type":"journal-article","created":{"date-parts":[[2020,5,20]],"date-time":"2020-05-20T08:02:29Z","timestamp":1589961749000},"page":"792-800","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Mesenchymal stem cells repair bone marrow damage of aging rats and regulate autophagy and aging genes"],"prefix":"10.1002","volume":"38","author":[{"given":"Zhihong","family":"Wang","sequence":"first","affiliation":[{"name":"Provincial Clinical Medical College of Fujian Medical University  Fuzhou China"},{"name":"Department of Hematology Fujian Provincial Hospital  Fuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yibin","family":"Shi","sequence":"additional","affiliation":[{"name":"Provincial Clinical 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