{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T16:39:34Z","timestamp":1789663174751,"version":"4.0.1"},"reference-count":32,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2020,6,23]],"date-time":"2020-06-23T00:00:00Z","timestamp":1592870400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["2171001"],"award-info":[{"award-number":["2171001"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:sec><jats:label\/><jats:p>During the development of primary Sj\u00f6gren's syndrome (pSS), aberrant expression of autoantigen is a hallmark event. To explore the regulation of autoantigen tripartite motif containing 21 (Ro\/SSA, TRIM21), microRNA profiling was performed in our previous study. In which, two <jats:italic>TRIM21<\/jats:italic>\u2010targeting microRNAs were identified, namely miR\u20101207\u20105p and miR\u20104695\u20103p. To further pursue their roles in the development of pSS, assays were performed with cultured human submandibular gland (HSG) cells, and salivary gland tissues. Results showed that transfection of miR\u20101207\u20105p or miR\u20104695\u20103p mimics down\u2010regulated not only the expression of <jats:italic>TRIM21<\/jats:italic>, but also the levels of pro\u2010apoptotic genes B cell lymphoma 2 associated X (<jats:italic>BAX<\/jats:italic>), Caspase 9 (<jats:italic>CASP\u20109<\/jats:italic>) and Caspase 8 (<jats:italic>CASP\u20108<\/jats:italic>). This finally led to antiapoptotic phenotypes in HSG cells. Consistent with the antiapoptotic activity, transfection of microRNA inhibitors up\u2010regulated the expression of <jats:italic>TRIM21<\/jats:italic> and led to a pro\u2010apoptotic phenotype. These therefore propose miR\u20101207\u20105p and miR\u20104695\u20103p as two antiapoptotic microRNAs functioning through apoptosis pathway. Supporting this speculation, assays performed with salivary gland tissues revealed down\u2010regulation of miR\u20101207\u20105p and miR\u20104695\u20103p, as well as up\u2010regulation of <jats:italic>TRIM21<\/jats:italic> and pro\u2010apoptotic <jats:italic>CASP\u20108<\/jats:italic> gene in pSS samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Significance of the study<\/jats:title><jats:p>For pSS patients, apoptosis of acinar and ductal epithelial cells has been proposed to be a potential mechanism that impairs the secretion of salivary glands. In our study, two autoantigen\u2010targeting microRNAs were characterized as antiapoptotic microRNAs functioning through apoptosis pathway, which may be potential targets for the treatment of pSS.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbf.3569","type":"journal-article","created":{"date-parts":[[2020,6,23]],"date-time":"2020-06-23T18:30:16Z","timestamp":1592937016000},"page":"1111-1118","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Characterization of antiapoptotic <scp>microRNAs<\/scp> in primary Sj\u00f6gren's syndrome"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5277-1155","authenticated-orcid":false,"given":"Ying","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Stomatology, Beijing Friendship Hospital Capital Medical University  Beijing 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