{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T10:01:02Z","timestamp":1782554462970,"version":"3.54.5"},"reference-count":13,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012537","name":"Ditmanson Medical Foundation Chia-Yi Christian Hospital","doi-asserted-by":"publisher","award":["R108"],"award-info":[{"award-number":["R108"]}],"id":[{"id":"10.13039\/501100012537","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:sec><jats:label\/><jats:p>Lung cancer is one of the leading causes of cancer\u2010related death worldwide. The most common type of lung cancer is non\u2010small cell lung cancer (NSCLC). When NSCLC is detected, patients are typically already in a metastatic stage. Metastasized cancer is a major obstacle of effective treatment and understanding the mechanisms underlying metastasis is critical to treat cancer. Herein, we selected an invasive subpopulation from the human lung cancer cell line A549 using the transwell system and named it as A549\u2010I5. Invasive and migratory activities of this cell line were analysed using wound healing, invasion, and migration assays. In addition, epithelial\u2010mesenchymal transition (EMT) markers, such as Snail 1, Twist, Vimentin, N\u2010cadherin and E\u2010cadherin, were assessed through immunoblotting. In comparison to A549 cells, the invasive A549\u2010I5 lung cancer cells had enhanced invasiveness, motility and EMT marker expression. Proteomic analysis identified 83 significantly differentially expressed proteins in A549\u2010I5 cells. These identified proteins were classified according to their cellular functions and most were involved in cytoskeleton, redox regulation, protein degradation and protein folding. In summary, our results provide potential diagnostic markers and therapeutic candidates for the treatment of NSCLC metastasis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Significance of the study<\/jats:title><jats:p>When NSCLC is detected, most patients are already in a metastatic stage. Herein, we selected an invasive subpopulation from a human lung cancer cell line which had increased EMT markers as well as high wound healing, invasion and migration abilities. Proteomic analysis identified numerous proteins associated with functions in cytoskeleton, redox regulation, protein degradation and protein folding that were differentially expressed in these cells. These results may provide potential diagnostic markers and therapeutic candidates for the treatment of NSCLC metastasis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbf.3599","type":"journal-article","created":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T22:40:31Z","timestamp":1604270431000},"page":"367-379","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Biomarker discovery in highly invasive lung cancer cell through proteomics approaches"],"prefix":"10.1002","volume":"39","author":[{"given":"Chin\u2010Ho","family":"Kuo","sequence":"first","affiliation":[{"name":"Division of Hematology\u2010Oncology, Department of Internal Medicine Ditmanson Medical Foundation Chia\u2010Yi Christian Hospital  Chiayi Taiwan"},{"name":"Department of Cosmetology and Health Care Min\u2010Hwei Junior College of Health Care Management  Tainan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng\u2010Wei","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Medical Science and Institute of Bioinformatics and Structural Biology National Tsing Hua University  Hsinchu Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Wei","family":"Hsu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Ditmanson Medical Foundation Chia\u2010Yi Christian Hospital  Chiayi Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsiu\u2010Chuan","family":"Chou","sequence":"additional","affiliation":[{"name":"Institute of Analytical and Environmental Sciences National Hsinchu University of Education  Hsinchu Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li\u2010Hsun","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Medical Science and Institute of Bioinformatics and Structural Biology National Tsing Hua University  Hsinchu Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ching\u2010Hsuan","family":"Law","sequence":"additional","affiliation":[{"name":"Department of Medical Science and Institute of Bioinformatics and Structural Biology National Tsing Hua University  Hsinchu Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Chieh","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Medical Science and Institute of Bioinformatics and Structural Biology National Tsing Hua University  Hsinchu Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u2010Yu","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Medical Science and Institute of Bioinformatics and Structural Biology National Tsing Hua University  Hsinchu 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