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Therefore, this study aimed to investigate the effect of spermidine, which is known as an inhibitor of ageing\u2010related oxidized proteins, on melanogenesis. Even though spermidine above 50\u2009\u03bcM had no effect on antioxidant activity and DOPA oxidation, it displayed tyrosinase activity. However, spermidine at 2000\u2009\u03bcM was cytotoxic in B16F1 cells using MTT assay. Spermidine above 125\u2009\u03bcM decreased the amount of intracellular hydrogen peroxide in a concentration\u2010dependent manner in DCFH\u2010DA analysis. It was also found that spermidine above 2000\u2009\u03bcM increased melanin synthesis in living cells. However, spermidine above 1000\u2009\u03bcM increased melanin synthesis in a concentration\u2010dependent manner in H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010treated B16F1 cells. Furthermore, spermidine enhanced the expression of tyrosine hydroxylase via MITF transcription factor involved in melanogenesis in H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010treated B16F1 cells. Therefore, these results suggest that spermidine could be applied as a potential stimulator of melanin synthesis for the prevention of hair greying.<\/jats:p>","DOI":"10.1002\/cbf.3619","type":"journal-article","created":{"date-parts":[[2021,1,25]],"date-time":"2021-01-25T20:45:55Z","timestamp":1611607555000},"page":"536-545","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Spermidine promotes melanin production through an <scp>MITF<\/scp> signalling pathway"],"prefix":"10.1002","volume":"39","author":[{"given":"Seong\u2010Hee","family":"Kang","sequence":"first","affiliation":[{"name":"Department of Applied Chemistry Dong\u2010Eui University  Busan South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5887-266X","authenticated-orcid":false,"given":"Moon\u2010Moo","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Applied Chemistry 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