{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T23:49:54Z","timestamp":1779148194288,"version":"3.51.4"},"reference-count":72,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,7,22]],"date-time":"2021-07-22T00:00:00Z","timestamp":1626912000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["51873052"],"award-info":[{"award-number":["51873052"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:sec><jats:label\/><jats:p>Increasing attention has been paid on the application of biodegradable materials such as magnesium and its alloys in neuron repair. AZ91D magnesium alloy coated with carbon nanotubes (CNTs) and\/or calcium phosphate (CaP)\/chitosan (CS) was fabricated in this study. To evaluate the bioactivity of these AZ91D\u2010based composites, the extracts were prepared by immersing samples in modified simulated body fluid (m\u2010SBF) for 0, 2, 8, 16, 24, 34, 44, 60, or 90\u2009days. Immunofluorescence staining for neuronal class III \u03b2\u2010tubulin (TUJ1) revealed that both CNTs\u2010CaP\/CS\u2010AZ91D and CaP\/CS\u2010AZ91D extracts promoted axon outgrowth of dorsal root ganglia (DRG) neurons, accompanied with increased expression of phosphorylated focal adhesion kinase (p\u2010FAK) and growth associated protein\u201043 (GAP\u201043). Besides, the extracts increased the expression and the release of neurotrophic factors including nerve growth factor (NGF) and brain\u2010derived neurotrophic factor (BDNF). ERK signalling was activated in DRG neurons after treating with either CNTs\u2010CaP\/CS\u2010AZ91D or CaP\/CS\u2010AZ91D extracts, and its inhibition with U0126 counteracted the beneficial effects of these extracts on DRG neuron. Overall, the extracts from these AZ91D\u2010based composites might promote DRG neuron growth via activating ERK signalling pathway. Notably, CNTs\u2010CaP\/CS\u2010AZ91D extracts showed a better promoting effect on neuron growth than CaP\/CS\u2010AZ91D. Assessment of ion elements showed that the addition of CNTs coating enhanced magnesium corrosion resistance and reduced the deposition of calcium and phosphorus on the surface of CaP\/CS\u2010AZ91D alloy. These findings demonstrate that CNTs\u2010CaP\/CS\u2010AZ91D likely provide a more suitable environment for neuron growth, which suggests a potential implantable biomaterial for the treatment of nerve injury.<\/jats:p><\/jats:sec><jats:sec><jats:title>Significance<\/jats:title><jats:p>AZ91D magnesium alloy coated with carbon nanotubes (CNTs) and\/or calcium phosphate (CaP)\/chitosan (CS) was fabricated and their immersion extracts were prepared using modified simulated body fluid in this study. Both extracts from CNTs\u2010CaP\/CS and CaP\/CS\u2010coated AZ91D magnesium alloy promotes rat dorsal root ganglia (DRG) neuron growth via activating ERK signalling pathway. Notably, the addition of CNTs improves the performance of CaP\/CS\u2010AZ91D. For the first time, our research demonstrates that CNTs\u2010CaP\/CS\u2010AZ91D likely provide a suitable environment for neuron growth, suggesting these AZ91D\u2010based composites as potential implantable biomaterials for the treatment of nerve injury.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbf.3662","type":"journal-article","created":{"date-parts":[[2021,7,23]],"date-time":"2021-07-23T01:04:52Z","timestamp":1627002292000},"page":"908-920","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["<scp>CNTs\u2010CaP<\/scp>\/chitosan\u2010coated <scp>AZ91D<\/scp> magnesium alloy extract promoted rat dorsal root ganglia neuron growth via activating <scp>ERK<\/scp> signalling pathway"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4055-9250","authenticated-orcid":false,"given":"Tingjiao","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Neurology First Affiliated 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