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Hepcidin activity is largely determined by its expression, which is regulated at the transcriptional level. Hepcidin transcription is induced not only by the iron status\u2010related bone morphogenetic protein (BMP)\u20102\/6, but also by inflammatory cytokines, such as interleukin (IL)\u20101\u03b2 and IL\u20106. The present study reveals that the microphthalmia (MiT)\/transcription factor E (TFE) family members are novel regulators of hepcidin transcription. Melanocyte\u2010inducing transcription factor (MITF)\u2010A, a member of the MiT\/TFE family, was identified as a positive regulator of hepcidin transcription via stimulus screening for transcription regulators. An E\u2010box (5\u2032\u2010CATGTG\u20103\u2032) spanning nt\u2010645 to nt\u2010640 of the murine hepcidin promoter was identified as an MITF\u2010A\u2010responsive element. Responsiveness to MITF\u2010A on hepcidin transcription decreased when the cells were stimulated with BMP2 or IL\u20101\u03b2. These results suggest a functional interaction between the MITF pathway and BMP\u2010 or IL\u20101\u03b2\u2010mediated signaling. TFEB and TFE3, members of the MiT\/TFE family, also stimulated hepcidin transcription, but the main region responsible for hepcidin transcription was distinct from that induced by MITF\u2010A. The region spanning nt\u2010581 to nt\u2010526 was involved in TFEB\/TFE3\u2010mediated hepcidin transcription. Considering that members of the MiT\/TFE family act as regulators of starvation\u2010induced lysosomal biogenesis, hepcidin expression may be controlled by additional pathways apart from those identified so far.<\/jats:p>","DOI":"10.1002\/cbf.3739","type":"journal-article","created":{"date-parts":[[2022,9,5]],"date-time":"2022-09-05T06:39:42Z","timestamp":1662359982000},"page":"742-749","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Transcriptional activation of hepcidin by the microphthalmia\/transcription factor E family"],"prefix":"10.1002","volume":"40","author":[{"given":"Manami","family":"Matsumura","sequence":"first","affiliation":[{"name":"Division of Applied Biosciences, Graduate School of Agriculture Kyoto University Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaru","family":"Murakami","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology Azabu 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