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One of the treatment strategies is islet isolation, which may damage these cells as they lack vasculature. Biocompatible scaffolds are one of the efficient techniques for dealing with this issue. The current study is aimed to determine the effect of transfected BM\u2010MSCS with angiomiR\u2010126 and \u2010210 on the survival and functionality of islets loaded into a 3D scaffold via laminin (LMN). AngiomiRs\/Poly Ethylenimine polyplexes were transfected into bone marrow\u2010mesenchymal stem cells (BM\u2010MSCs), followed by 3\u2010day indirect co\u2010culturing with islets laden in collagen (Col)\u2010based hydrogel scaffolds containing LMN. Islet proliferation and viability were significantly increased in LMN\u2010containing scaffolds, particularly in the miRNA\u2010126 treated group. <jats:italic>Insulin<\/jats:italic> gene expression was superior in Col scaffolds, especially, in the BM\u2010MSCs\/miRNA\u2010126 treated group. <jats:italic>VEGF<\/jats:italic> was upregulated in the LMN\u2010containing scaffolds in both miRNA\u2010treated groups, specifically in the miRNA\u2010210, leading to VEGF secretion. MiRNAs'\u00a0target genes showed no downregulation in LMN\u2010free scaffolds; while a drastic downregulation was seen in the LMN\u2010containing scaffolds. The highest insulin secretion was recorded in the Oxidized dextran (Odex)\/Col<jats:sup>LMN+<\/jats:sup> group with miRNA\u2010126. LMN\u2010containing biocompatible scaffolds, once combined with angiomiRs and their downstream effectors, promote islets survival and restore function, leading to enhanced angiogenesis and glycemic status.<\/jats:p>","DOI":"10.1002\/cbf.3781","type":"journal-article","created":{"date-parts":[[2023,2,23]],"date-time":"2023-02-23T07:36:46Z","timestamp":1677137806000},"page":"296-308","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Indirect co\u2010culture of islet cells in 3D biocompatible collagen\/laminin scaffold with angiomiRs transfected mesenchymal stem cells"],"prefix":"10.1002","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5737-0323","authenticated-orcid":false,"given":"Fatemeh","family":"Sabet Sarvestani","sequence":"first","affiliation":[{"name":"Transplant Research Center Shiraz University of Medical Sciences Shiraz 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