{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T05:54:39Z","timestamp":1786254879339,"version":"build-2736575974"},"reference-count":46,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,3,14]],"date-time":"2023-03-14T00:00:00Z","timestamp":1678752000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The current study aimed to investigate the inhibitory activity of aqueous extracts of different plant parts of <jats:italic>Rosa damascena<\/jats:italic>, represented by the whole rose, petals, and calyces, against clinical isolates of <jats:italic>Staphylococcus spp<\/jats:italic>., <jats:italic>Escherichia coli<\/jats:italic>, and <jats:italic>Klebsiella pneumoniae<\/jats:italic>, and the inhibition of the bacterial cell.\u00a0The isolates were obtained from the advanced microbiology laboratory for postgraduate studies in the Department of Biology, Faculty of Education for Girls. They were isolated from urinary tract infections, which were subsequently subjected to diagnosis by the Vitek\u20102 compact system to confirm the type of bacteria as well as their sensitivity to antibiotics. The results obtained included <jats:italic>Staphylococcus aureus, Staphylococcus haemolyticus, Staphylococcus lentus, Staphylococcus saprophyticus, E. coli<\/jats:italic>, and <jats:italic>K. pneumoniae<\/jats:italic>.\u00a0A test was conducted to investigate the microbiological inhibitory activity of aqueous plant extracts of the whole rose, petals, and calyces using the well diffusion method and three concentrations of each aqueous extract (25, 50, and 100\u2009mg\/ml). The results showed the inhibitory ability of all concentrations of the different extracts toward\u00a0<jats:italic>Staphylococcus spp<\/jats:italic>., and <jats:italic>E. coli<\/jats:italic> and <jats:italic>K. pneumoniae<\/jats:italic> bacteria were not affected by the different concentrations of the plant extract. The concentration of (100\u2009mg\/ml) for the aqueous extract was the most efficient in inhibiting growth compared to the other concentrations.\u00a0The synergistic effect of three antibiotics was examined (Amoxicillin\u2010clavulanate 10\/20\u2009\u03bcg, Piperacillin 100\u2009\u03bcg, Trimethoprim\u2010sulfamethoxazole 23.75\/1.25\u2009\u03bcg) and for all concentrations of the aqueous plant extract was investigated in both <jats:italic>E. coli<\/jats:italic> and <jats:italic>K. pneumoniae<\/jats:italic>, as it found a synergistic action between some of the antibiotics and extracts towards inhibiting the growth of the two bacterial isolates Resistance to the plant extract alone.\u00a0Bacterial isolates showed a significant decrease in the rate of adhesion to epithelial cells isolated from urine samples of healthy women in the presence of the aqueous extract of whole rose, petals, and calyces at their three concentrations compared with the control treatment.<\/jats:p>","DOI":"10.1002\/cbf.3788","type":"journal-article","created":{"date-parts":[[2023,3,15]],"date-time":"2023-03-15T01:22:37Z","timestamp":1678843357000},"page":"365-374","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Aqueous <i>Rosa damascena<\/i> extract: Antibacterial activity and its role of adhesion to human epithelial cells in vitro"],"prefix":"10.1002","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3961-5726","authenticated-orcid":false,"given":"Alaa","family":"Alsafi","sequence":"first","affiliation":[{"name":"Department of Biology, Faculty of Education for Girls Kufa University Kufa Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siham Jasim","family":"AlKaabi","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Education for Girls Kufa University Kufa Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,3,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-021-03249-7"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf9286"},{"key":"e_1_2_9_4_1","first-page":"1","article-title":"Antimicrobial resistance: tackling a crisis for the health and wealth of nations","author":"O'neill J","year":"2014","journal-title":"Rev Antimicrob Resist"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.3103"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-16807-0"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcme.2015.09.005"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2022.114984"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/jphp.13185"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/4543723"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/medicina55060289"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eursup.2016.04.002"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00470-13"},{"issue":"10","key":"e_1_2_9_14_1","first-page":"957","article-title":"Effect of rose demascena extracts in prolonging the duration of the preservation of yoghurt","volume":"9","author":"Al\u2010Musawi AT","year":"2018","journal-title":"Indian J Public Health Res Dev"},{"key":"e_1_2_9_15_1","volume-title":"Basic laboratory procedures in clinical bacteriology","author":"Vandepitte J","year":"2003"},{"key":"e_1_2_9_16_1","unstructured":"CLSI.Performance Standards for Antimicrobial Susceptibility Testing; Twentieth informational supplement. 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