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However, the role and impact of fibrosis during the early stages and development of cachexia in under\u2010investigated. The purpose of this study was to determine if fibrosis occurs during cachexia development, and to evaluate this in both sexes. Female and male C57BL6\/J mice were injected with phosphate\u2010buffered saline or Lewis Lung Carcinoma (LLC) at 8\u2010week of age, and tumors were allowed to develop for 1, 2, 3, or 4 weeks. 3wk and 4wk female tumor\u2010bearing mice displayed a dichotomy in tumor growth and were reassigned to high tumor (HT) and low tumor (LT) groups. In vitro analyses were also performed on cocultured C2C12 and 3T3 cells exposed to LLC conditioned media. Immunohistochemistry and quantitative polymerase chain reaction (qPCR) analysis were used to investigate fibrosis and fibrosis\u2010related signaling in skeletal muscle. Collagen deposition in skeletal muscle was increased in the 1wk, LT, and HT groups in female mice. However, collagen deposition was only increased in the 4wk group in male mice. In general, female mice displayed earlier alterations in extracellular matrix (ECM)\u2010related genes beginning at 1wk post\u2010LLC injection. Whereas this was not seen in males. While overall tumor burden is tightly correlated to cachexia development in both sexes, fibrotic development is not. Male mice did not exhibit early\u2010stage alterations in ECM\u2010related genes contrary to what was noted in female mice.<\/jats:p>","DOI":"10.1002\/cbf.3797","type":"journal-article","created":{"date-parts":[[2023,5,8]],"date-time":"2023-05-08T00:21:44Z","timestamp":1683505304000},"page":"478-489","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Development of skeletal muscle fibrosis in a rodent model of cancer cachexia"],"prefix":"10.1002","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1919-5907","authenticated-orcid":false,"given":"Tyrone A.","family":"Washington","sequence":"first","affiliation":[{"name":"Exercise Muscle Biology Laboratory University of Arkansas Fayetteville Arkansas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleanor R.","family":"Schrems","sequence":"additional","affiliation":[{"name":"Exercise Muscle Biology Laboratory University of Arkansas 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