{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T00:56:04Z","timestamp":1760316964290,"version":"build-2065373602"},"reference-count":36,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,5,24]],"date-time":"2023-05-24T00:00:00Z","timestamp":1684886400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Inflammation is an innate reaction of the body of an individual when subjected to the noxious factors repeatedly. Pharmacological approaches focused at disrupting cytokine signaling networks have become significant therapeutic alternatives for the treatment of inflammatory illnesses, cancer and autoimmune disorders. High levels of inflammatory mediators, particularly interleukin IL\u20101, IL\u20106, IL\u201018, IL\u201012, and tumor necrosis factor alpha leads to a cytokine storm in the body. Among all the released cytokines in a patient suffering from inflammatory disorder, IL\u20106 mediator has a pivotal role in this inflammatory cascade which progresses to a cytokine storm. Therefore, the blockage of the IL\u20106 inflammatory mediator could be a promising treatment option for the patients with hyper inflammatory conditions. The phytochemicals could provide the new lead compounds against the IL\u20106 mediator. <jats:italic>Ficus carica<\/jats:italic> has been the ideal plant of research and investigation due to its commercial, economic and medical importance. The anti\u2010inflammatory properties of <jats:italic>F. carica<\/jats:italic> were further investigated by in silico and in vivo approaches. The docking scores of Cyanidin\u20103,5\u2010diglucoside, Kaempferol\u20107\u2010O\u2010rutinoside, Cyanidin\u20103\u2010rhamnoglucoside, and Rutin are \u22129.231, \u22128.921, \u22128.840, and \u22128.335 Kcal\/mole respectively. The free energy of binding and stability of the docked complexes of these top four phytochemicals with the IL\u20106 were further analyzed by Molecular Mechanics\u2010Generalized Born Surface Area and Molecular Dynamic simulations, respectively. The in vivo anti\u2010inflammatory carrageenan\u2010induced rat paw edema model was used for the validation of in silico results. The maximum percentage paw edema inhibition with petroleum ether and ethyl acetate was 70.32% and 45.05%, respectively. The in vivo anti\u2010inflammatory activity confirms the anti\u2010inflammatory potential of <jats:italic>F. carica<\/jats:italic>. Therefore, it is predicted that Cyanidin\u20103,5\u2010diglucoside, Kaempferol\u20107\u2010O\u2010rutinoside, Cyanidin\u20103\u2010rhamnoglucoside, and Rutin have the potential to inhibit the IL\u20106 mediator which will aid in mitigating the cytokine storm in patients with acute inflammations.<\/jats:p>","DOI":"10.1002\/cbf.3813","type":"journal-article","created":{"date-parts":[[2023,5,24]],"date-time":"2023-05-24T08:09:36Z","timestamp":1684915776000},"page":"573-589","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The inhibitory potential of chemical constituents of <i>Ficus carica<\/i> targeting interleukin\u20106 (IL\u20106) mediated inflammation"],"prefix":"10.1002","volume":"41","author":[{"given":"Hussaam Ul Haq","family":"Mansoor","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy, Punjab University College of Pharmacy University of the Punjab  Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5849-1365","authenticated-orcid":false,"given":"Abrar","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Punjab University College of Pharmacy University of the Punjab  Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatima","family":"Rasool","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Punjab University College of Pharmacy University of the Punjab  Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,5,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-018-0739-1"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.03.006"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07201"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0b013e3181c9e31d"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1017\/CBO9781139195737"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000001452"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/BOR.0000000000000255"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/1744666X.2021.1919086"},{"issue":"3","key":"e_1_2_8_10_1","first-page":"25","article-title":"Antibody\u2010dependent enhancement of SARS coronavirus infection and its role in the pathogenesis of SARS","volume":"22","author":"Yip MS","year":"2016","journal-title":"Hong Kong medical J"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-020-0110-5"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/324073a0"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a016295"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3153"},{"key":"e_1_2_8_15_1","doi-asserted-by":"crossref","unstructured":"ZhouY FuB ZhengX et al.Aberrant pathogenic GM\u2010CSF+T cells and inflammatory CD14+ CD16+ monocytes in severe pulmonary syndrome patients of a new coronavirus.2020. 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