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Recently, studies have highlighted the antineoplastic potential of natural compounds, such as polyphenols, in the adjuvant therapy context to treat CM. The objective of the present study was to evaluate the effect of different concentrations of curcumin (0.1\u2013100\u2009\u00b5M) on the metastatic CM cell line SK\u2010MEL\u201028. The cells were treated for 6 and 24\u2009h with different concentrations of curcumin. Cell viability was assessed by 3\u2010(4,5\u2010dimethyl\u20102thiazolyl)\u20102,5\u2010diphenyl\u20102H\u2010tetrazolium bromide (MTT) assay and fluorescence microscopy. The apoptotic\u2010inducing potential was detected by annexin V flow cytometry. The wound healing assay was used to verify cell migration after the curcumin exposition. The redox profile was evaluated by levels of the pro\u2010oxidant markers reactive oxygen species (ROS) and Nitric oxide (NOx) and antioxidants of total thiols (PSH) and nonprotein thiols. The gene expression and enzymatic activity of caspase 3 were evaluated by reverse transcription\u2010quantitative polymerase chain reaction and a sensitive fluorescence assay, respectively. Curcumin significantly decreased the cell viability of SK\u2010MEL\u201028 cells at both exposure times. It also induced apoptosis at the highest concentration tested (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u2009.0001). SK\u2010MEL\u201028 cell migration was inhibited by curcumin after treatment with 10\u2009\u00b5M (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u2009.0001) and 100\u2009\u00b5M (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u2009.0001) for 6 and 24\u2009h (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u2009.0006 and\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u2009.0001, respectively\n                    <jats:italic>)<\/jats:italic>\n                    . Furthermore, curcumin significantly increased levels of ROS and NOx. Finally, curcumin was capable of increasing the gene expression at 10\u2009\u00b5M (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u2009.0344) and 100\u2009\u00b5M (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u2009.0067) and enzymatic activity at 10\u2009\u00b5M (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u2009.0086) and 100\u2009\u00b5M (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u2009.0001) of caspase 3 after 24\u2009h. For the first time, we elucidated in our study that curcumin increases ROS levels, promoting oxidative stress that activates the caspase pathway and culminates in SK\u2010MEL\u201028 metastatic CM cell death.\n                  <\/jats:p>","DOI":"10.1002\/cbf.3863","type":"journal-article","created":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T11:15:45Z","timestamp":1696418145000},"page":"1295-1304","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Curcumin promotes apoptosis of human melanoma cells by caspase 3"],"prefix":"10.1002","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3126-954X","authenticated-orcid":false,"given":"Daiane","family":"Manica","sequence":"first","affiliation":[{"name":"Postgraduate Programme in Biochemistry Federal University of Santa Catarina Florian\u00f3polis Santa Catarina Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilnei Bruno da","family":"Silva","sequence":"additional","affiliation":[{"name":"Multicentric Postgraduate Programme in Biochemistry and Molecular Biology State University of Santa Catarina  Lages Santa Catarina Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alana Patr\u00edcia da","family":"Silva","sequence":"additional","affiliation":[{"name":"Postgraduate Programme in Biochemistry Federal University of Santa Catarina Florian\u00f3polis Santa Catarina Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filomena","family":"Marafon","sequence":"additional","affiliation":[{"name":"Postgraduate Programme in Biochemistry Federal University of Santa Catarina Florian\u00f3polis Santa Catarina Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah Franco Vieira de Oliveira","family":"Maciel","sequence":"additional","affiliation":[{"name":"Postgraduate Programme in Biomedical Sciences Federal University of Fronteira Sul  Chapec\u00f3 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