{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T20:02:46Z","timestamp":1766088166308,"version":"build-2065373602"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,1,24]],"date-time":"2024-01-24T00:00:00Z","timestamp":1706054400000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mammalian sperm remain quiescent but fertile for several weeks in cauda epididymis. Although several sperm quiescent factors of epididymal plasma have been identified in goat, pig and cattle; however, little is known in sheep. In the present study, purification and characterization of a novel sperm quiescent protein of ovine cauda epididymal plasma (CEP) was carried out. The sperm quiescent protein was partially purified by hydroxyapatite gel adsorption chromatography followed by DEAE\u2010sepharose\u00ae anion exchange chromatography. In the latter, the sperm quiescent activity was eluted both in 0.05 and 0.2\u2009M potassium phosphate buffer (pH 7.5) fractions having a predominant protein of about 80 and 70\u2009kDa with 87% and 63% homogeneity, respectively. The proteins were designated as motility\u2010inhibitory factor of sheep I and II (MIFS\u2010I and II), respectively. Significant (about 60%) inhibition of sperm motility was observed following treatment of cauda epididymal sperm with 6 and 12\u2009\u00b5g\/mL of partially purified MIFS\u2010I and II, respectively. Specific activities of the partially purified MIFS\u2010I and II were 563 and 261\u2009U\/mg\u00a0of protein, while the fold\u2010purification of the activity were 5119 and 2373, respectively. Both the proteins were heat\u2010labile and the activity was completely lost following incubation at 100\u00b0C for 5\u2009min. Further, the partially purified MIFS\u2010I (5\u2009\u00b5g\/mL) caused significant reduction in in vitro sperm capacitation and slight decline in tyrosine phosphorylated p72 and p52 proteins; however the protein was nontoxic to sperm. Mass spectrometric analysis of MIFS\u2010I revealed significant identity with human semaphorin 3D. Both dot blot and western blot analysis demonstrated cross\u2010reactivity of MIFS\u2010I with polyclonal anti\u2010human SEMA3D antibody. It was concluded that the MIFS\u2010I of ovine CEP was putative ovine semaphorin 3D protein having potent sperm quiescent and decapacitating activities and it possibly acts through inhibition of protein tyrosine phosphorylation.<\/jats:p>","DOI":"10.1002\/cbf.3930","type":"journal-article","created":{"date-parts":[[2024,1,24]],"date-time":"2024-01-24T05:53:49Z","timestamp":1706075629000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Purification and partial characterization of a sperm motility\u2010inhibitory protein of ram cauda epididymal plasma"],"prefix":"10.1002","volume":"42","author":[{"given":"Pyare","family":"Lal","sequence":"first","affiliation":[{"name":"Department of Veterinary Biochemistry, College of Veterinary &amp; Animal Science Rajasthan University of Animal and Veterinary Sciences  Bikaner Rajasthan India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kalpana","family":"Jorasia","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biochemistry, College of Veterinary &amp; Animal Science Rajasthan University of Animal and Veterinary Sciences  Bikaner Rajasthan India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Narendra Singh","family":"Rathore","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biochemistry, College of Veterinary &amp; Animal Science Rajasthan University of Animal and Veterinary Sciences  Bikaner Rajasthan India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vijay","family":"Kumar","sequence":"additional","affiliation":[{"name":"Division of Animal Physiology &amp; Biochemistry ICAR\u2010Central Sheep and Wool Research Institute  Avikanagar Rajasthan 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