{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T17:18:29Z","timestamp":1765041509305,"version":"build-2065373602"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,2,11]],"date-time":"2024-02-11T00:00:00Z","timestamp":1707609600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study uncovered the potential clinical value and molecular driving mechanisms of circular RNAs (circRNAs) in gallbladder cancer (GBC). Differentially expressed circRNAs in GBC cells were screened by high\u2010throughput sequencing. CircRNA_CDKN1A (circBase ID: hsa_circ_0076194) was knocked out in BGC\u2010SD cells through transfection with sh\u2010circRNA_CDKN1A. Then, proliferation was investigated via CCK8 and EdU assays, apoptosis via flow cytometry, migration via wound healing assays, and invasion via Transwell assays. Bioinformatics analysis of circRNA_CDKN1A\u2010related signaling pathways was performed using MetScape and g:Profiler. Results showed that the knockdown of circRNA_CDKN1A enhanced the proliferation, migration, and invasion of GBC cells and inhibited apoptosis. In addition, knocking out circRNA_CDKN1A promoted GBC cell proliferation and enhanced the dry indices of the OCT4 protein and CD34 expression levels. The knockdown of circRNA_CDKN1A activated the epithelial\u2013mesenchymal transition pathway. Bioinformatics analysis revealed that the biological role of circRNA_CDKN1A in GBC cells involved the NF\u2010\u03baB pathway. LY2409881, which is an NF\u2010\u03baB inhibitor, reversed the effects induced by the knockdown of circRNA_CDKN1A in GBC\u2010SD cells. In summary, the knockdown of circRNA_CDKN1A promoted the progression of GBC by activating the NF\u2010\u03baB signaling pathway. For the first time, this study revealed the mechanism of circRNA_CDKN1A\u2010mediated regulatory action in GBC and identified the newly discovered circRNA_CDKN1A\u2013NF\u2010\u03baB signaling axis as a potentially important candidate for clinical therapy and prognostic diagnosis of GBC.<\/jats:p>","DOI":"10.1002\/cbf.3952","type":"journal-article","created":{"date-parts":[[2024,2,12]],"date-time":"2024-02-12T00:07:36Z","timestamp":1707696456000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Downregulated circRNA_CDKN1A promotes gallbladder cancer progression through activation of the NF\u2010\u03baB pathway"],"prefix":"10.1002","volume":"42","author":[{"given":"Bin","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Gastroenterology and Pancreatic Surgery The Affiliated Jiangning Hospital of Nanjing Medical University Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Medical Imaging, Nanjing Chest Hospital The Affiliated Nanjing Brain Hospital of Nanjing Medical University Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Medical Imaging, Nanjing Chest Hospital The Affiliated Nanjing Brain Hospital of Nanjing Medical University Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Medical Imaging, Nanjing Chest Hospital The Affiliated Nanjing Brain Hospital of Nanjing Medical University Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rongping","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Pancreatic Surgery The Affiliated Jiangning Hospital of Nanjing Medical University Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0913-0652","authenticated-orcid":false,"given":"Dong","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Pancreatic Surgery The Affiliated Jiangning Hospital of Nanjing Medical University Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,2,11]]},"reference":[{"issue":"1","key":"e_1_2_9_2_1","first-page":"31","article-title":"Application and research progress of organoids in cholangiocarcinoma and gallbladder carcinoma","volume":"11","author":"Huang YP","year":"2021","journal-title":"Am J Cancer 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