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Betaine is a trimethyl glycine with antioxidant, antidiabetic, and anti\u2010inflammatory potential. The aim of this study is to investigate the potential of betaine on the expression of aging and oxidative stress markers in ovarian and testicular cells under hyperglycemic conditions. Testicular and ovarian cells were subjected to four different conditions, including normal glucose and hyperglycemia, with or without betaine (5\u2009mM). The cells with hyperglycemia saw an increase in malondialdehyde (MDA), methylglyoxal (MGO), expression of a receptor for AGE, and aging\u2010related genes (\u03b2\u2010GAL), and a decrease in the activity of antioxidant enzymes including catalase, glutathione peroxidase, and superoxide dismutase. The treatment with betaine, in contrast, decreased the amount of MGO and MDA, and also downregulated aging\u2010related signaling. Although hyperglycemia induces senescence in testicular and ovarian cells, the use of betaine may have a protective effect against the cell senescence, which may be useful in the management of infertility.<\/jats:p>","DOI":"10.1002\/cbf.3973","type":"journal-article","created":{"date-parts":[[2024,3,16]],"date-time":"2024-03-16T02:48:40Z","timestamp":1710557320000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Betaine postpones hyperglycemia\u2010related senescence in ovarian and testicular cells: Involvement of RAGE and \u03b2\u2010galactosidase"],"prefix":"10.1002","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-0173-5634","authenticated-orcid":false,"given":"Neda","family":"Mohammadi","sequence":"first","affiliation":[{"name":"Department of Clinical Biochemistry, School of Medicine Zanjan University of Medical Sciences Zanjan 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