{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T00:38:54Z","timestamp":1760315934348,"version":"build-2065373602"},"reference-count":52,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,3,15]],"date-time":"2024-03-15T00:00:00Z","timestamp":1710460800000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Fluoride (F) has been employed worldwide to control dental caries. More recently, it has been suggested that the consumption of low doses of F in the drinking water may reduce blood glucose levels, introducing a new perspective for the use of F for the management of blood glucose. However, the exact mechanism by which F affects blood glucose levels remains largely unexplored. Given that the small gut plays a pivotal role in glucose homeostasis, the aim of this study was to investigate the proteomic changes induced by low doses of F in the ileum of female nonobese\u2010diabetic (NOD) mice. Forty\u2010two female NOD mice were divided into two groups based on the F concentration in their drinking water for 14 weeks: 0 (control) or 10\u2009mgF\/L. At the end of the experimental period, the ileum was collected for proteomic and Western blot analyses. Proteomic analysis indicated an increase in isoforms of actin, gastrotropin, several H2B histones, and enzymes involved in antioxidant processes, as well as a decrease in enzymes essential for energy metabolism. In summary, our data indicates an adaptive response of organism to preserve protein synthesis in the ileum, despite significant alterations in energy metabolism typically induced by F, therefore highlighting the safety of controlled fluoridation in water supplies.<\/jats:p>","DOI":"10.1002\/cbf.3976","type":"journal-article","created":{"date-parts":[[2024,3,16]],"date-time":"2024-03-16T02:54:04Z","timestamp":1710557644000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Adaptive responses of the ileum of NOD mice to low\u2010dose fluoride: A proteomic exploratory study"],"prefix":"10.1002","volume":"42","author":[{"given":"Juliana S.","family":"Trevizol","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry University of S\u00e3o Paulo Bauru Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathalia R.","family":"Buzalaf","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry University of S\u00e3o Paulo Bauru Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aline","family":"Dionizio","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry University of S\u00e3o Paulo Bauru Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aislan Q.","family":"Delgado","sequence":"additional","affiliation":[{"name":"Institute of Biosciences S\u00e3o Paulo State University Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o P. Z.","family":"de Lara","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry University of S\u00e3o Paulo Bauru Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana C.","family":"Magalh\u00e3es","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry University of S\u00e3o Paulo Bauru Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 R.","family":"Bosqueiro","sequence":"additional","affiliation":[{"name":"Department of Physical Education, Faculty of Science S\u00e3o Paulo State University Bauru Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5985-3951","authenticated-orcid":false,"given":"Mar\u00edlia A. R.","family":"Buzalaf","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry University of S\u00e3o Paulo Bauru Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,3,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0722.1996.tb00104.x"},{"key":"e_1_2_9_2_2","unstructured":"Discussion423\u2010415 430\u2010412."},{"issue":"6","key":"e_1_2_9_3_1","article-title":"Water fluoridation for the prevention of dental caries","author":"Iheozor\u2010Ejiofor Z","year":"2015","journal-title":"Cochrane Database Syst Rev"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000325107"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2018.09.010"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0158121"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0075343"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-018-1542-4"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2010.07.011"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-01090-y"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-21533-4"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2021.129607"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02405334"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/119.10.1411"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0271-5317(05)80826-7"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1159\/000325146"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034515581186"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2018.10.055"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0106646"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2020.126602"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1590\/1678-7757-2023-0036"},{"issue":"12","key":"e_1_2_9_22_1","first-page":"241","article-title":"Ten great public health achievements\u2010\u2010United States, 1900\u20101999","volume":"48","author":"Centers for Disease Control and Prevention","year":"1999","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11883-017-0642-5"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm727"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1097\/MPA.0000000000000828"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345950740011201"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.140419"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1106"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034517750850"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2019.124357"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-018-1344-8"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac1052"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.42.29749"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200401265"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00430.2005"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2007.07.012"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0801194105"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmgh.2016.12.009"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2015.01.004"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhepr.2021.100387"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dsx.2021.102366"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.49078"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1530\/JOE-12-0385"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12016-014-8422-2"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/dmrr.559"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-006-0562-3"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2019.05.034"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1164097"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408444.2022.2122771"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.etap.2020.103331"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.etap.2020.103511"}],"container-title":["Cell Biochemistry and Function"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbf.3976","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T13:45:36Z","timestamp":1760276736000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/cbf.3976"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3]]},"references-count":52,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,3]]}},"alternative-id":["10.1002\/cbf.3976"],"URL":"https:\/\/doi.org\/10.1002\/cbf.3976","archive":["Portico"],"relation":{},"ISSN":["0263-6484","1099-0844"],"issn-type":[{"type":"print","value":"0263-6484"},{"type":"electronic","value":"1099-0844"}],"subject":[],"published":{"date-parts":[[2024,3]]},"assertion":[{"value":"2024-01-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e3976"}}