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These actinobacteria's silver nanoparticles were synthesized and characterized using transmission electron microscopy (TEM) and UV\u2010Visible spectroscopy. Anticancer was measured using the MTT test, reactive oxygen species (ROS) generation measured with DCFDA, mitochondrial membrane potential (MMP) measurement, and DAPI fluorescence intensity activity was measured in treated and non\u2010treated cancerous cells. The IC<jats:sub>50<\/jats:sub> value for 5\u2010FU (a), LA2(O) (b), LA2(R) (c), LA2(ON) (d), and LA2(RN) (e) was obtained at 3.91\u2009\u03bcg\/mL (52.73% cell viability), 56.12\u2009\u03bcg\/mL (52.35% cell viability), 44.90\u2009\u03bcg\/mL (52.3% cell viability), 3.45\u2009\u03bcg\/mL (50.25% cell viability), and 8.05\u2009\u03bcg\/mL (48.72% cell viability), respectively. TEM micrographs revealed discrete, well\u2010separated AgNPs particles of size 7.88\u2009\u00b1\u20092 to 12.86\u2009\u00b1\u20090.24\u2009nm. Gas chromatography\u2010mass spectrometry was also performed to detect the compounds in bioactive metabolites where n\u2010hexadecanoic acid was obtained as the most significant one. MTT test showed a substantial decline in A549 cell viability (up to 48.72%), 2.75\u2010fold increase in ROS generation was noticed in comparison to untreated A549 lung cancer cells when measured with DCFDA. A total of 0.31\u2010fold decrease in MMP and 1.74\u2010fold increase in DAPI fluorescence intensity compared to untreated A549 lung cancer cells suggests that the synthesized nanoparticles promote apoptosis in cancerous cells. Our findings suggests that the secondary metabolites of <jats:italic>M. proteolyticum<\/jats:italic> and <jats:italic>S. rochei<\/jats:italic> in nanoparticle form can be used as a significant compound against lung cancers.<\/jats:p>","DOI":"10.1002\/cbf.3988","type":"journal-article","created":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T03:06:20Z","timestamp":1711508780000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Malignance\u2010restriction activity exhibited by bioactive compounds of selected actinobacteria as silver nanoparticles against A549 lung cancer cell lines"],"prefix":"10.1002","volume":"42","author":[{"given":"Naushin","family":"Bano","sequence":"first","affiliation":[{"name":"Department of Bioengineering Integral University Lucknow Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anamika","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Chemistry Aligarh Muslim University Aligarh Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Amir","sequence":"additional","affiliation":[{"name":"Department of Bioengineering Integral University Lucknow Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohd. 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