{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T04:19:51Z","timestamp":1788927591262,"version":"build-2803163510"},"reference-count":27,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,3,19]],"date-time":"2024-03-19T00:00:00Z","timestamp":1710806400000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004912","name":"Sichuan University","doi-asserted-by":"publisher","award":["2022NSFSC0627"],"award-info":[{"award-number":["2022NSFSC0627"]}],"id":[{"id":"10.13039\/501100004912","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MOST 106\u2010 2811\u2010B\u2010650\u2010003"],"award-info":[{"award-number":["MOST 106\u2010 2811\u2010B\u2010650\u2010003"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The majority of adenocarcinoma lung cancer is found in nonsmokers. A history of tobacco use is more common in squamous cell carcinoma of the lung. The aim of this study is to identify the cisplatin (CDDP)\u2010resistance that promotes lung squamous carcinoma cell growth through nicotine\u2010mediated HDAC1\/7nAchR\/E2F\/pRb cell cycle activation. Squamous cell carcinoma (NCI\u2010H520 and NCI\u2010H157) cells were examined after cisplatin and nicotine treatment by 3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyl\u20102H\u2010tetrazolium bromide assay, cell migration assay, immunofluorescence staining, western blot analysis, and immunoprecipitation analysis. Consequently, CDDP is released from DNA and Rb phosphorylated pRb as a result of nicotine\u2010induced cancer cell proliferation through 7nAchR, which then triggers the opening of the HDAC1 cell cycle. The cell cycle is stopped when CDDP adducts are present. Nicotine exerts cancer cytoprotective effects by allowing HDAC1 repair mechanisms to re\u2010establish E2F promoting DNA stimulation cell cycle integrity in the cytosol and preventing potential CDDP and HDAC1 suppressed in the nuclear. Concentration expression of nicotine causes squamous carcinoma cell carcinogens to emerge from inflammation. COX2, NF\u2010KB, and NOS2 increase as a result of nicotine\u2010induced squamous carcinoma cell inflammation. Nicotine enhanced the cell growth\u2010related proteins such as \u03b17nAchR, EGFR, HDAC1, Cyclin D, Cyclin E, E2F, Rb, and pRb by western blot analysis. It also induced cancer cell inflammation and growth. As a result, we suggest that nicotine will increase the therapeutic resistance effects of CDDP. This has the potential to interact with nicotine through \u03b17nAchR receptors and HDAC1\/Cyclin D\/E2F\/pRb potentially resulting in CDDP therapy resistance, as well as cell cycle\u2010induced cancer cell growth.<\/jats:p>","DOI":"10.1002\/cbf.3990","type":"journal-article","created":{"date-parts":[[2024,3,20]],"date-time":"2024-03-20T01:25:32Z","timestamp":1710897932000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Cisplatin\u2010resistance induces lung squamous carcinoma cell growth by nicotine\u2010mediated \u03b17nAchR\/HDAC1\/Cyclin D1\/pRb cell cycle activation"],"prefix":"10.1002","volume":"42","author":[{"given":"Leh","family":"Rao","sequence":"first","affiliation":[{"name":"Department of Medical Technology Shaoguan University  Shaoguan City Guangdong Province People's Republic of China"},{"name":"Department of Biomedicine Chengdu Medical College  Chengdu People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Medical Technology Shaoguan University  Shaoguan City Guangdong Province People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0349-3632","authenticated-orcid":false,"given":"Jia\u2010Ping","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Medical Technology Shaoguan University  Shaoguan City Guangdong Province People's Republic of 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