{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T00:33:46Z","timestamp":1760315626439,"version":"build-2065373602"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,4,13]],"date-time":"2024-04-13T00:00:00Z","timestamp":1712966400000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Toxicity caused by carbon tetrachloride (CCl<jats:sub>4<\/jats:sub>) can lead to serious liver injury. The aim of the study is to investigate the protective effects of oregano oil (<jats:italic>Origanum minutiflorum<\/jats:italic> extract oil) against CCl<jats:sub>4<\/jats:sub>\u2010induced liver injury. Two doses of oregano oil were used in the experiment: a low dose (LD; 20\u2009mg\/kg) and a high dose (HD; 60\u2009mg\/kg) during 2 weeks. CCl<jats:sub>4<\/jats:sub> caused severe liver damage, nucleolus destruction in hepatocytes and cytogenetic changes in the nucleus. Indirectly, CCl<jats:sub>4<\/jats:sub> causes decreased protein synthesis and significantly high creatinine and urea values. Hematological disorders have been recorded, such as decreased RBC and hemoglobin concentration, increased WBC and deformability of the erythrocyte membrane. Both doses of oregano oil had protective effects. Improved protein synthesis and high globulins level, creatinine and urea were found in both groups. Cytogenetic changes in the nucleus of hepatocytes were reduced. A high dose of oregano oil had maximal protective effects for RBC, but a very weak effect on hemoglobin synthesis. Also, WBC and lymphocyte values were low. Origanum stimulates protein synthesis and recovery of hepatocytes after liver injury, reduces the deformability of the erythrocyte membrane. High doses of oregano oil decreased WBC and lymphocytes which may lead to a weakening of the immune response. However, high doses are more effective against severe platelet aggregation than low doses, suggesting an effective treatment against thrombocytosis.<\/jats:p>","DOI":"10.1002\/cbf.4015","type":"journal-article","created":{"date-parts":[[2024,4,13]],"date-time":"2024-04-13T03:04:25Z","timestamp":1712977465000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Therapeutic benefit of oregano oil in the acute idiosyncratic hepatotoxicity induced by carbon tetrachloride in rats: Adverse effects of high dose of oreganum"],"prefix":"10.1002","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8854-7478","authenticated-orcid":false,"given":"Damir","family":"Suljevi\u0107","sequence":"first","affiliation":[{"name":"Faculty of Science, Department of Biology, Sarajevo, Bosnia and Herzegovina University of Sarajevo Sarajevo Bosnia and Herzegovina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1281-1527","authenticated-orcid":false,"given":"Muhamed","family":"Fo\u010dak","sequence":"additional","affiliation":[{"name":"Faculty of Science, Department of Biology, Sarajevo, Bosnia and Herzegovina University of Sarajevo Sarajevo Bosnia and Herzegovina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0194-2334","authenticated-orcid":false,"given":"Rifat","family":"\u0160krijelj","sequence":"additional","affiliation":[{"name":"Faculty of Science, Department of Biology, Sarajevo, Bosnia and Herzegovina University of Sarajevo Sarajevo Bosnia and Herzegovina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6398-5677","authenticated-orcid":false,"given":"Maja","family":"Mitra\u0161inovi\u0107\u2010Bruli\u0107","sequence":"additional","affiliation":[{"name":"Faculty of Science, Department of Biology, Sarajevo, Bosnia and Herzegovina University of Sarajevo Sarajevo Bosnia and Herzegovina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,4,13]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0042-107437"},{"key":"e_1_2_10_3_1","doi-asserted-by":"crossref","first-page":"124","DOI":"10.25004\/IJPSDR.2009.010301","article-title":"A comprehensive review of different liver toxicants used in experimental pharmacology","volume":"1","author":"Bigoniya P","year":"2009","journal-title":"Inter J Pharm Sci Drug Res"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1750"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200110716"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/1283824"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/713611034"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/209036a0"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2011.193"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/00984100050082085"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxics6020025"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-8206.2008.00618.x"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfda.2013.09.012"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf049859g"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.6785"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21249653"},{"key":"e_1_2_10_17_1","first-page":"563","article-title":"Therapeutic potential of Origanum vulgare leaf hydroethanolic extract against renal oxidative stress and nephrotoxicity induced by paraquat in rats","volume":"9","author":"Sharifi\u2010Rigi A","year":"2019","journal-title":"Avicenna J Phytomed"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1080\/13813455.2018.1437186"},{"issue":"1","key":"e_1_2_10_19_1","first-page":"279","article-title":"Therapeutic effects of royal jelly on some biochemical parameters in nephrotoxicity induced by carbon tetrachloride (CCl4) in albino rats","volume":"13","author":"Ali F","year":"2021","journal-title":"Egypt Acad J Biol Sci B Zool"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.3829"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2017.01.001"},{"key":"e_1_2_10_22_1","first-page":"45","article-title":"Origanum Majoranum extract modulates gene expression, hepatic and renal changes in a rat model of type 2 diabetes","volume":"15","author":"Soliman MM","year":"2016","journal-title":"Iran J Pharm Res"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202200948"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.3906\/zoo-1706-18"},{"volume-title":"Kidney, Reference Module in Biomedical Sciences","year":"2022","author":"Rankin GO","key":"e_1_2_10_25_1"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-08-046884-6.01008-3"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2014.05.014"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.21702"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1177\/0960327111429140"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6882-11-96"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1155\/2007\/23296"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1080\/1745039X.2013.858897"},{"key":"e_1_2_10_33_1","article-title":"Effects of two sources of Mexican oregano oil on performance, blood profile, carcass variables, and meat of broilers","volume":"47","author":"Silva\u2010V\u00e1zquez R","year":"2018","journal-title":"Rev Brasil Zoot"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00709-012-0431-5"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.3109\/13880209.2014.908399"}],"container-title":["Cell Biochemistry and Function"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbf.4015","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T12:11:40Z","timestamp":1760271100000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/cbf.4015"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4]]},"references-count":34,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["10.1002\/cbf.4015"],"URL":"https:\/\/doi.org\/10.1002\/cbf.4015","archive":["Portico"],"relation":{},"ISSN":["0263-6484","1099-0844"],"issn-type":[{"type":"print","value":"0263-6484"},{"type":"electronic","value":"1099-0844"}],"subject":[],"published":{"date-parts":[[2024,4]]},"assertion":[{"value":"2024-01-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e4015"}}