{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T09:28:11Z","timestamp":1789464491853,"version":"build-2803163510"},"reference-count":56,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,6,6]],"date-time":"2024-06-06T00:00:00Z","timestamp":1717632000000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004697","name":"Hamadan University of Medical Sciences","doi-asserted-by":"publisher","award":["140012039867"],"award-info":[{"award-number":["140012039867"]}],"id":[{"id":"10.13039\/501100004697","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease. Metabolic and mitochondrial dysregulation are critical causal factors in the pathogenesis and progression of RA. Mitochondrial dysfunction include abnormal energy metabolism, and excessive production of reactive oxygen species (ROS). This study aimed to investigate the adenosine triphosphate (ATP), mitochondrial membrane potential (\u0394\u03a8m), ROS, and mRNA expression level of <jats:italic>ROMO1<\/jats:italic> (as ROS modulator) and <jats:italic>OMA1<\/jats:italic> (as regulator mitochondrial dynamics) of peripheral blood mononuclear cells (PBMC) in RA patients. The study participants were 50 patients with RA and 50 sex\u2010 and age\u2010matched healthy volunteers. PBMC of all participant were isolated by Ficoll\u2010Paque. Alteration in \u0394\u03a8m and cellular ROS were measured using flow cytometry, ATP level was also assessed via luminometry, and <jats:italic>ROMO1<\/jats:italic> and <jats:italic>OMA1<\/jats:italic> mRNA expression via qRT\u2010PCR assay. A significant decrease in ATP (<jats:italic>p<\/jats:italic>\u2009=\u2009.005) and \u0394\u03a8m (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001) was observed in the PBMC of RA compared to control. The ROS levels were significantly higher in the PBMC of RA compared to the control (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001). <jats:italic>ROMO1<\/jats:italic> and <jats:italic>OMA1<\/jats:italic> mRNA expression was also significantly increased in RA patients compared to control (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001). The decrease in ATP is strongly associated with ROS increasing in PBMC of RA patients, denoting an inverse and negative relationship between ATP and ROS production. Also, a decrease in \u0394\u03a8m was observed. It seems that in line with mitochondrial dysfunction in PBMC, increased expression of <jats:italic>ROMO1<\/jats:italic> and <jats:italic>OMA1<\/jats:italic> genes could also be involved in the development of RA.<\/jats:p>","DOI":"10.1002\/cbf.4025","type":"journal-article","created":{"date-parts":[[2024,6,6]],"date-time":"2024-06-06T23:37:59Z","timestamp":1717717079000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Peripheral blood mononuclear cells show markers of mitochondrial dysfunction in rheumatoid arthritis"],"prefix":"10.1002","volume":"42","author":[{"given":"Anahita","family":"Abbasifard","sequence":"first","affiliation":[{"name":"Department of Clinical Biochemistry, School of Medicine Hamadan University of Medical Sciences Hamadan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghasem","family":"Solgi","sequence":"additional","affiliation":[{"name":"Department of Immunology, School of Medicine Hamadan University of Medical Sciences Hamadan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9048-4528","authenticated-orcid":false,"given":"Iraj","family":"Khodadadi","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, School of Medicine Hamadan University of Medical Sciences Hamadan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amin","family":"Doosti\u2010Irani","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, School of Health Hamadan University of Medical Sciences Hamadan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7243-660X","authenticated-orcid":false,"given":"Heidar","family":"Tayebinia","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, School of Medicine Hamadan University of Medical Sciences Hamadan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5441-8304","authenticated-orcid":false,"given":"Alireza","family":"Fazaeli","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Rheumatology Hamadan University of Medical Sciences Hamadan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,6,6]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41413-018-0016-9"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00296-020-04731-0"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0152925"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/keab014"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22020686"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2018.1"},{"key":"e_1_2_9_8_1","first-page":"161","article-title":"Peripheral blood mononuclear cells","author":"Kleiveland CR","year":"2015","journal-title":"Impact Food Bioact Health"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.673916"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm9030740"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom12091216"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2020.10.025"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.4078\/jrd.2016.23.6.340"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.26356"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222212411"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12029-019-00330-w"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.4068\/cmj.2019.55.3.136"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.06.140"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2008.02.098"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.26508\/lsa.202201767"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2012.70"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.202050827"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.27584"},{"issue":"10055","key":"e_1_2_9_24_1","first-page":"2023","article-title":"IainB. 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