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Male Swiss albino mice were exposed to 0.2\u2009Gy\u2009gamma radiation\/week for four consecutive weeks then injected intraperitoneally (i.p) with a single dose of 8.3 \u00d7 10<jats:sup>6<\/jats:sup> CFU <jats:italic>Escherichia coli<\/jats:italic>\/g b.w. then injected i.p. with 1.0\u2009mg\/kg cholecalciferol (Vit D<jats:sub>3)<\/jats:sub> for 7 days starting 4 h after <jats:italic>E. coli<\/jats:italic> injection. The results revealed that Cholecalciferol and low dose gamma radiation caused significant depletion in the severity of <jats:italic>E. coli<\/jats:italic> infection (colony forming unit per milliliter), log<jats:sub>10<\/jats:sub> of <jats:italic>E. coli<\/jats:italic>, Tumor necrosis factor alpha, Interleukin 6, VEGF, alanine aminotransferase, and aspartate aminotransferase levels and significant elevation in IL\u201010, IL\u20104, and HO\u20101. Immunohistochemical analysis of caspase\u20103 expression in lung tissue section showed low caspase\u20103 expression in cholecalciferol and low dose gamma radiation treated group. Histopathological examinations were performed in both lung and liver tissues which also emphasis the biochemical findings. Our results exhibit the importance of cholecalciferol and low dose gamma radiation in improving liver function and providing anti\u2010inflammatory response in diseases causing cytokine storm.<\/jats:p>","DOI":"10.1002\/cbf.4026","type":"journal-article","created":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T00:34:21Z","timestamp":1714610061000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Cytokine storm modulation using cholecalciferol and low dose gamma radiation in <i>Escherichia coli<\/i> infected mice"],"prefix":"10.1002","volume":"42","author":[{"given":"Gehan R.","family":"Abdel\u2010Hamid","sequence":"first","affiliation":[{"name":"Radiation Biology, National Center for Radiation Research and Technology Egyptian Atomic Energy Authority Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2520-9017","authenticated-orcid":false,"given":"Dalia M.","family":"Mostafa","sequence":"additional","affiliation":[{"name":"Radiation Biology, National Center for Radiation Research and Technology Egyptian Atomic Energy Authority Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rasha M.","family":"Fathy","sequence":"additional","affiliation":[{"name":"Drug Radiation Research, National Center for Radiation Research and Technology Egyptian Atomic Energy Authority Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dina M.","family":"Lotfy","sequence":"additional","affiliation":[{"name":"Drug Radiation Research, National Center for Radiation Research and Technology Egyptian Atomic Energy Authority Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soheir","family":"Osman","sequence":"additional","affiliation":[{"name":"Radiation Biology, National Center for Radiation Research and Technology Egyptian Atomic Energy Authority Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,5]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222313009"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1188\/10.CJON.E10-E21"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.11.025"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40425-018-0343-9"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.13.040195.001343"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12072-018-9911-9"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00228-010-0862-x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2FO01221A"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.4067\/S0716-10182019000400447"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-017-0639-8"},{"key":"e_1_2_8_12_1","first-page":"51","article-title":"Burn and trauma associated lung injury","author":"Xia Z\u2010F","year":"2020","journal-title":"Spri Nat Singapore Pte Ltd"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-020-01947-6"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10030515"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/CM9.0000000000002425"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1079\/BJN2003837"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0131740"},{"issue":"292","key":"e_1_2_8_18_1","article-title":"Putative roles of vitamin D in modulating immune response and immunopathology associated with COVID\u201019","volume":"15","author":"Kumar R","year":"2020","journal-title":"J. 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