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These natural bioactive compounds have sparked growing interest in employing medicinal plants for addressing various conditions, such as inflammatory diseases, diabetes, and cancer. This study focuses on assessing the qualitative phytochemical composition, antioxidant potential, and cytotoxic effects of blueberry (<jats:italic>Vaccinium sect. Cyanococcus<\/jats:italic>) extract using three different solvents, namely water, ethanol, and methanol. The extract exhibited notable antioxidant activities, as evidenced by DPPH and H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> free radical scavenging assays. The cell viability assay also demonstrated cell growth inhibition in A549 cells. Furthermore, nine specific phytocompounds sourced from existing literature were selected for molecular docking studies against CDK6 and, AMPK key protein kinases which enhance the cancer progression. The molecular docking results also revealed favorable binding scores, with a high score of \u22129.5\u2009kcal\/mol in CDK6 protein and a maximum score of AMPK with targets of \u22128.8\u2009kcal\/mol. The selected phytocompounds\u2019 pharmacodynamic properties such as ADMET also supported the study. Furthermore, rutin stated that pre\u2010dominantly present in blueberry plants shows a potent cytotoxicity effect in A549 cells. Functional annotations by bioinformatic analysis for rutin also revealed the strong enrichment in the involvement of PI3K\/AKT1\/STAT, and p53 signaling pathways. Based on this analysis, the identified rutin and other compounds hold a promising anticancer activity. Overall, the comprehensive evaluation of both in vitro and in silico data suggests that the <jats:italic>Vaccinium sect. Cyanococcus<\/jats:italic> extract could serve as a valuable source of pharmaceutical agents and may prove effective in future therapeutic applications.<\/jats:p>","DOI":"10.1002\/cbf.4027","type":"journal-article","created":{"date-parts":[[2024,5,7]],"date-time":"2024-05-07T23:44:25Z","timestamp":1715125465000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Blueberry extract and its bioactive compounds mitigate oxidative stress and suppress human lung cancer cell (A549) growth by modulating the expression of p53\/EGFR\/STAT3\/IL6\u2010mediated signaling molecules"],"prefix":"10.1002","volume":"42","author":[{"given":"Kalaiselvi","family":"Krishnamoorthy","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Centre of Molecular Medicine and Diagnostics (COMManD), Saveetha Institute of Medical and Technical Sciences, Saveetha Dental College and Hospitals Saveetha University Chennai 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