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The dried aerial parts of <jats:italic>A. officinalis<\/jats:italic> L. were extracted with methanol. Total phenolic and flavonoid content was analyzed. Antioxidant and antimicrobial effects were tested against both gram\u2010positive and gram\u2010negative bacteria. Gas chromatography\u2010mass spectrometry analysis revealed the presence of 10 phytochemical compounds, and cyclobutane (26.07%) was identified as the major photochemical compound. The methanol extract exhibited the maximum amount of total phenolic content (118.24\u2009\u00b1\u20094.42\u2009mg QE\/g dry weight of the dry extract) (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup>\u2009=\u20090.994) and the total flavonoid content was 94\u2009\u00b1\u20092.34\u2009mg QE\/g dry weight of the dry extract (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup>\u2009=\u20090.999). The IC<jats:sub>50<\/jats:sub> value for 2,2\u2032\u2010azino\u2010bis (3\u2010ethylbenzothiazoline\u20106\u2010sulfonic acid was 107.12\u2009\u00b1\u20093.42\u2009\u03bcg\/mL, and it was high for 1,1\u2010diphenyl\u20102\u2010picryl hydrazyl (123.94\u2009\u00b1\u20092.31\u2009\u03bcg\/mL). The IC<jats:sub>50<\/jats:sub> value was 72.49\u2009\u00b1\u20093.14 against HepG2 cell lines, and a decreased value was obtained (102.54\u2009\u00b1\u20094.17\u2009g\/mL) against MCF\u20107 cell lines. The methanol extract increased the expression of caspase mRNA and Bax mRNA levels when compared to the control experiment (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). The conclusions, <jats:italic>A. officinalis<\/jats:italic> L. aerial parts extract exhibited antibacterial, antifungal, and antioxidant activities.<\/jats:p>","DOI":"10.1002\/cbf.4093","type":"journal-article","created":{"date-parts":[[2024,7,9]],"date-time":"2024-07-09T01:33:10Z","timestamp":1720488790000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["In vitro and in silico evaluation of bioactivities and chemical composition of the aerial parts of <i>Anchusa officinalis<\/i> L. methanol extract"],"prefix":"10.1002","volume":"42","author":[{"given":"Reem M.","family":"Aljowaie","sequence":"first","affiliation":[{"name":"Department of Botany and Microbiology College of Science, King Saud University Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mashail Fahad","family":"Alsayed","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology College of Science, King Saud University Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noorah A.","family":"Alkubaisi","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology College of Science, King Saud University Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Omer M.","family":"Almarfadi","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy College of Pharmacy, King Saud University Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed A.","family":"Farrag","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology College of Science, King Saud University Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adel A.","family":"Abdulmanea","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology College of Science, King Saud University Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akram A.","family":"Alfuraydi","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology College of Science, King Saud University Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tarad","family":"Abalkhail","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology College of Science, King Saud University Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mourad A. M.","family":"Aboul\u2010Soud","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Sciences College of Applied Medical Sciences, King Saud University Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6970-5207","authenticated-orcid":false,"given":"Ibrahim M.","family":"Aziz","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology College of Science, King Saud University Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,7,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13237-022-00405-3"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/bjbs.2023.11387"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph10094274"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/app132413196"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/app10134597"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cis.2018.03.001"},{"key":"e_1_2_10_8_1","first-page":"Ch. 9","volume-title":"Natural Medicinal Plants","author":"Fongang Fotsing Yannick S","year":"2021"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2023.116981"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2011.574802"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.15244\/pjoes\/171298"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.31893\/multiscience.2024026"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25163625"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsps.2020.08.001"},{"key":"e_1_2_10_15_1","unstructured":"McLooneFS. 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