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This major contradiction may lead to inconsistent and misleading toxicology due to the limited biological representation of these assays. Cytotoxicity evaluation is a crucial element of screening the biological response to new biomaterials. However, as standard test methods do not recapitulate the in vivo environment, tailored adaptations may be required to reflect the true biological response elicited toward\u00a0novel biomaterials.<\/jats:p>","DOI":"10.1002\/cbf.4097","type":"journal-article","created":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T00:48:41Z","timestamp":1721090921000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["PLGA\u2010PEG\u2010PLGA hydrogels induce cytotoxicity in conventional in vitro assays"],"prefix":"10.1002","volume":"42","author":[{"given":"Chloe L.","family":"Stewart","sequence":"first","affiliation":[{"name":"School of Pharmacy University of Nottingham Nottingham UK"},{"name":"Institute of Developmental and Regenerative Medicine University of Oxford Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew L.","family":"Hook","sequence":"additional","affiliation":[{"name":"School of Pharmacy University of Nottingham Nottingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mischa","family":"Zelzer","sequence":"additional","affiliation":[{"name":"School of Pharmacy University of Nottingham Nottingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Marlow","sequence":"additional","affiliation":[{"name":"School of Pharmacy University of Nottingham Nottingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7588-0415","authenticated-orcid":false,"given":"Anna M.","family":"Piccinini","sequence":"additional","affiliation":[{"name":"School of Pharmacy University of Nottingham Nottingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,7,15]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsps.2019.08.001"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/polym14122379"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S356453"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.23735"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:PHAM.0000008050.99985.5c"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-323-91952-4.00020-9"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11040727"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25112505"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2024.113795"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics13020234"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.3144\/expresspolymlett.2016.34"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1134\/S0965545X12080020"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1177\/0192623318810199"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-409X(01)00242-3"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-3659(01)00276-0"}],"container-title":["Cell Biochemistry and Function"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbf.4097","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T12:38:59Z","timestamp":1760272739000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/cbf.4097"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7]]},"references-count":15,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2024,7]]}},"alternative-id":["10.1002\/cbf.4097"],"URL":"https:\/\/doi.org\/10.1002\/cbf.4097","archive":["Portico"],"relation":{},"ISSN":["0263-6484","1099-0844"],"issn-type":[{"value":"0263-6484","type":"print"},{"value":"1099-0844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7]]},"assertion":[{"value":"2024-03-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e4097"}}