{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T13:13:11Z","timestamp":1782738791648,"version":"3.54.5"},"reference-count":42,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T00:00:00Z","timestamp":1726099200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>In this study, the protective effects of <jats:italic>Panax notoginseng<\/jats:italic> saponins (PNS) against gamma radiation\u2010induced DNA damage and associated physiological alterations in Swiss albino mice were investigated. Exposure to gamma radiation led to a dose\u2010dependent increase in cytokinesis\u2010blocked micronuclei (CBMN) double\u2010strand DNA breaks (DSBs), dicentric aberrations (DC), formation in peripheral blood mononuclear cells. However, pretreatment with PNS at concentrations of 1, 5, and 10\u2009\u00b5g\/mL significantly attenuated the frequencies of DC and CBMN in a concentration\u2010dependent manner. PNS administration before radiation exposure also reduced radiation\u2010induced DSBs in BL, indicating protection against reactive oxygen species generation and DNA damage. Notably, pretreatment with PNS at 10\u2009\u00b5g\/mL prevented the overexpression of \u03b3\u2010H2AX, proteins associated with DNA damage response, in irradiated mice. In addition, in vivo studies showed intraperitoneal administration of PNS (25\u2009mg\/kg body weight) for 1\u2009h before radiation exposure mitigated lipid peroxidation levels and restored antioxidant status, countering oxidative damage induced by gamma radiation. Furthermore, PNS pretreatment reversed the decrease in hemoglobin (Hb) content, white blood cell count, and red blood cell count in irradiated mice, indicating preservation of hematological parameters. Overall, PNS demonstrated an anticlastogenic effect by modulating radiation\u2010induced DSBs and preventing oxidative damage, thus highlighting its potential as a protective agent against radiation\u2010induced DNA damage and associated physiological alterations. Clinically, PNS will be beneficial for cancer patients undergoing radiotherapy, but their pharmacological properties and toxicity profiles need to be studied.<\/jats:p>","DOI":"10.1002\/cbf.4115","type":"journal-article","created":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T10:45:53Z","timestamp":1726137953000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<i>Panax notoginseng<\/i> Saponins Ameliorate Gamma Radiation\u2010Mediated Damages in Human Peripheral Blood Monocytes and Swiss Albino Mice"],"prefix":"10.1002","volume":"42","author":[{"given":"Ming\u2010Yu","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Chinese Medicine Cangzhou Medical College Cangzhou 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