{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T18:44:45Z","timestamp":1776019485199,"version":"3.50.1"},"reference-count":62,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,10,18]],"date-time":"2024-10-18T00:00:00Z","timestamp":1729209600000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001843","name":"Science and Engineering Research Board","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001843","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Spermatogenesis and sperm maturation that occur in the testis and epididymis respectively are crucial for fertility. Factors secreted by the testicular and epididymal epithelial cells govern the processes of gametogenesis and maturation. Proteins encoded by the sperm\u2010associated antigen 11a (<jats:italic>Spag11a<\/jats:italic>) gene are implicated as having a possible role in sperm maturation. However, studies that demonstrate their definite role in fertility and sperm function using knockout models have not yet reported. In this study, <jats:italic>Spag11a<\/jats:italic> knockout mice were generated, genotyped and the reproductive parameters (fecundity, sperm count, capacitation, and acrosome reaction) and sperm proteome were determined. Litter size and sperm count were decreased in the <jats:italic>Spag11a<\/jats:italic> knockout mice when compared to the wild\u2010type controls. Spermatozoa from the knockout mice were able to undergo capacitation. However, acrosome reaction did not occur in sperm obtained from knockout mice. Structural abnormalities in the head and tail structures were evident in the spermatozoa of knockout mice. Perturbations in the expression of sperm proteins that are involved in gametogenesis were evident. The subfertility observed in <jats:italic>Spag11a<\/jats:italic> knockout mice could be a manifestation of lower sperm count, impaired acrosome reactions, and disturbances in the sperm proteome. The results of this study lend further support to the role of <jats:italic>Spag11a<\/jats:italic> gene in male gamete function.<\/jats:p>","DOI":"10.1002\/cbf.70003","type":"journal-article","created":{"date-parts":[[2024,10,19]],"date-time":"2024-10-19T00:54:28Z","timestamp":1729299268000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The Sperm\u2010Associated Antigen 11A (<i>Spag11a<\/i>) Knockout Mice Display Sub\u2010Fertility and Perturbations in the Sperm Proteome"],"prefix":"10.1002","volume":"42","author":[{"given":"Kumari","family":"Sangeeta","sequence":"first","affiliation":[{"name":"Department of Animal Biology University of Hyderabad  Hyderabad India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jamil","family":"Aisha","sequence":"additional","affiliation":[{"name":"Department of Animal Biology University of Hyderabad  Hyderabad 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