{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T00:42:49Z","timestamp":1760316169324,"version":"build-2065373602"},"reference-count":54,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,2,27]],"date-time":"2025-02-27T00:00:00Z","timestamp":1740614400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>As immune cells, neutrophils serve as the first line of defense against infections; however, the mechanism by which neutrophils regulate lipid metabolism is unknown. The neutrophil depletion group was treated with 100\u2009\u03bcg InVivoMAb anti\u2010mouse Ly6G 6 times, whereas the control group mice were intraperitoneally injected with the same quantity of InVivoMAb rat IgG2a. Body fat content, triglycerides (TGs), total cholesterol (TC), low\u2010density lipoprotein cholesterol (LDL\u2010C) in the jejunum and ileum, as well as 9 long\u2010chain fatty acids (LCFAs) in the intestinal contents were significantly decreased. Furthermore, genes involved in the absorption of lipids in each segment of the intestine also showed decreased expression. Neutrophil\u2010depletion and control models were administered 25\u2009\u03bcCi of <jats:sup>3<\/jats:sup>H\u2010cholesterol by gavage. The distribution of <jats:sup>3<\/jats:sup>H cholesterol in the intestinal segment, heart, liver, serum, and feces was not altered by anti\u2010Ly6G antibodies. Metagenomics was applied to investigate uncultured microorganisms in the intestinal contents to identify bacteria containing lipid metabolism genes. At the species level, 12 bacteria were involved in unsaturated LCFA synthesis, among which 2 increased and 10 decreased. The overall relative abundance of these bacteria decreased from 3.102% to 0.734%. Many genes involved in lipid metabolism were also reduced as a result, such as fatty acid synthase and peroxisome proliferator\u2010activated receptor \u03b3. In conclusion, neutrophil depletion does not affect intestinal lipid absorption in the diet but leads to a decrease in the overall relative abundance of gut bacteria involved in unsaturated LCFA synthesis. Consequently, intestinal lipid synthesis and absorption are reduced.<\/jats:p>","DOI":"10.1002\/cbf.70060","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T04:40:48Z","timestamp":1741668048000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Neutrophil Depletion Reduced the Relative Abundance of Unsaturated Long\u2010Chain Fatty Acid Synthesis Microbiota and Intestinal Lipid Absorption"],"prefix":"10.1002","volume":"43","author":[{"given":"Xingyu","family":"Lu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions Soochow University Suzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yike","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions Soochow University Suzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yitong","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions Soochow University Suzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang","family":"Li","sequence":"additional","affiliation":[{"name":"School of Biology &amp; Basic Medical Sciences Soochow University Suzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiong","family":"Feng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions Soochow University Suzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun","family":"Gao","sequence":"additional","affiliation":[{"name":"Health Management Center The First Affiliated Hospital of Soochow University Suzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Liu","sequence":"additional","affiliation":[{"name":"Health Management Center The First Affiliated Hospital of Soochow University Suzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Zhou","sequence":"additional","affiliation":[{"name":"Nutriology Department The First Affiliated Hospital of Soochow University Suzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haizhen","family":"Yang","sequence":"additional","affiliation":[{"name":"Health Management Center The Second Affiliated Hospital of Soochow University Suzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji","family":"Zhang","sequence":"additional","affiliation":[{"name":"Ophthalmology Department The Second Affiliated Hospital of Soochow University Suzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fengmei","family":"Cui","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions Soochow University Suzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5716-758X","authenticated-orcid":false,"given":"Qiu","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions Soochow University Suzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,2,27]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoncol.2019.0086"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2017.11.005"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.7150\/jca.32621"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.1050721"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2022.1050643"},{"issue":"3","key":"e_1_2_10_7_1","first-page":"298","article-title":"Retrospective Analyses of Serum Lipids and Lipoproteins and Severity of Disease in 60Co\u2010Irradiated Sus scrofa domestica and Macaca mulatta","volume":"57","author":"Parra N. C.","year":"2007","journal-title":"Comparative Medicine"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers14081932"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.34739"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-019-0547-0"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2022.100944"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M002774"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2023.01.009"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01493-18"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-019-0485-4"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2024.03.014"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2020.05.013"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-023-01655-2"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-018-0492-6"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.R800018-JLR200"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.ade0522"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/oby.23653"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.11.097"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.700534"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tjnut.2023.08.030"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tjnut.2022.12.009"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-020-02495-x"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/nqab081"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-37551-1"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2022.1037941"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22189929"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0284292"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2021.108927"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.200281"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-009-0608-y"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1997.273.2.G289"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12020-017-1323-4"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s44161-024-00462-8"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1160\/TH11-02-0096"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00041.2018"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12915-019-0715-8"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2020.05.013"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.916848"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2015.04.004"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0FO01939A"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aba8310"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06852"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.718460"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplegacy.1968.214.4.836"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplegacy.1969.216.4.915"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1139\/y63-177"},{"issue":"5","key":"e_1_2_10_52_1","first-page":"585","article-title":"Absorption of Amino Acids in Different Parts of the Small Intestine in Growing Pigs. IV. Effect of Sugars on Absorption of Amino Acids and Water","volume":"32","author":"Buraczewska L.","year":"1981","journal-title":"Acta Physiologica Polonica"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0083031"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12252"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43587-021-00086-8"}],"container-title":["Cell Biochemistry and Function"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbf.70060","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T14:24:46Z","timestamp":1760279086000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/cbf.70060"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,27]]},"references-count":54,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,3]]}},"alternative-id":["10.1002\/cbf.70060"],"URL":"https:\/\/doi.org\/10.1002\/cbf.70060","archive":["Portico"],"relation":{},"ISSN":["0263-6484","1099-0844"],"issn-type":[{"type":"print","value":"0263-6484"},{"type":"electronic","value":"1099-0844"}],"subject":[],"published":{"date-parts":[[2025,2,27]]},"assertion":[{"value":"2024-08-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70060"}}