{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T03:08:05Z","timestamp":1788577685510,"version":"build-2803163510"},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,3,20]],"date-time":"2025-03-20T00:00:00Z","timestamp":1742428800000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Cervical cancer (CC) is the second cause of death by a neoplasia in woman in Mexico. Among the factors that contribute to its development are prolonged infection by a high\u2010risk HPV type and the use of estrogens. It is well known that diagnosis at early stages is extremely important since, in most cases, progression towards carcinogenesis could be prevented, hence the importance of finding candidates that serve as early biomarkers. Several studies have shown that the expression level of the tumor suppressor miR\u2010218 is diminished in CC while oncomiR miR\u201021 is overexpressed. On the other hand, it has been reported that the Potassium calcium\u2010activated channel subfamily M alpha 1 (\n                    <jats:italic>Kcnma1<\/jats:italic>\n                    ) oncogene, a known target gene of miR\u2010218, is overexpressed in CC. However, there are few studies on the expression of this oncogene in Cervical Intraepithelial Neoplasia 1 (CIN 1). In this study, the analysis of the K14E7HPV16 carcinogenesis model in young mice (1.5\u2010month\u2010old), showed that a single\u2010dose of 17\u03b2\u2010estradiol (E\n                    <jats:sub>2<\/jats:sub>\n                    ) increased both the cell proliferation and the\n                    <jats:italic>Bcl\u20102<\/jats:italic>\n                    oncogene expression, as well as promoted the development of CIN 1. Interestingly, the hormonal stress and the E7 expression, favor the physiological response of the organism in transgenic young mice by decreasing the expression levels of the tumor suppressor miR\u2010218 and increasing the expression of the\n                    <jats:italic>Kcnma1<\/jats:italic>\n                    and\n                    <jats:italic>Bcl\u20102<\/jats:italic>\n                    mRNA oncogenes in both, cervical tissue and serum. This work demonstrates the significance of a single E\n                    <jats:sub>2<\/jats:sub>\n                    stimulation and the expression of the HPV E7 oncoprotein in the early stage of cervical carcinogenesis. In addition, we provide strong evidence about\n                    <jats:italic>Kcnma1<\/jats:italic>\n                    oncogene as a target gene of miR\u2010218 and that both could be used as early circulating biomarkers of CC.\n                  <\/jats:p>","DOI":"10.1002\/cbf.70065","type":"journal-article","created":{"date-parts":[[2025,3,23]],"date-time":"2025-03-23T04:00:41Z","timestamp":1742702441000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["E7HPV16 Oncogene and 17beta\u2010Estradiol Stress, Promotes Oncogenic microRNA Expression Patterns, Cell Proliferation and Cervical Intraepithelial Neoplasia 1"],"prefix":"10.1002","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9825-7426","authenticated-orcid":false,"given":"Erandi","family":"Arvizu\u2010Hernandez","sequence":"first","affiliation":[{"name":"Department of Genetics and Molecular Biology Centro deInvestigaci\u00f3n y de Estudios Avanzados del IPN Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4576-881X","authenticated-orcid":false,"given":"Rodolfo","family":"Ocadiz\u2010Delgado","sequence":"additional","affiliation":[{"name":"Department of Genetics and Molecular Biology Centro deInvestigaci\u00f3n y de Estudios Avanzados del IPN Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0024-7076","authenticated-orcid":false,"given":"Patricio","family":"Gariglio","sequence":"additional","affiliation":[{"name":"Department of Genetics and Molecular Biology Centro deInvestigaci\u00f3n y de Estudios Avanzados del IPN Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,20]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"Globocan. 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